Solary E, Guiguet M, Zeller V, Casasnovas R O, Caillot D, Chavanet P, Guy H, Mack G
Service d'Hématologie Clinique, C.H.U. Le Bocage, Dijon, France.
Am J Hematol. 1992 Mar;39(3):163-71. doi: 10.1002/ajh.2830390303.
Interleukin-6 (IL-6) was demonstrated to be a strong autocrine or paracrine plasmocytoma cell growth factor in humans. Using a bioassay, high serum IL-6 (S-IL-6) levels were correlated with disease severity in plasma cell dyscrasias. Since other cytokines could interfere with the bioassays, we developed a specific radioimmunoassay to study S-IL-6 levels in 102 patients with monoclonal gammopathy (MG). S-IL-6 level was studied by a double antibody radioimmunoassay using a rabbit polyclonal anti-IL-6 antibody and a human recombinant IL-6 as the standard. The lowest value of the standard significantly different from zero was found to be 78 pg/ml. Within-run and between-run precisions were characterized by a mean coefficient of variation of 3.72 and 5.5%, respectively. The mean analytical recovery was found to be 113% and the immunochemical identity of IL-6 standard and S-IL-6 was shown by dilution tests. IL-6 was detected in all tested sera. Sera from 66 healthy volunteers and 43 patients with acute leukemia or malignant lymphoma were tested as controls. In healthy subjects, S-IL-6 values were 294 +/- 86 pg/ml. MG were classified as multiple myeloma (MM), macroglobulinemia, and MG of undetermined significance (MGUS). The distribution of S-IL-6 levels in patients with MG was significantly higher than in healthy subjects but lower than in patients with acute leukemia or Hodgkin's lymphoma. Results obtained in 55 patients with MM were related to other biological parameters. S-IL-6 levels correlated with bone-marrow plasmacytosis (P less than .0005), serum-lactate dehydrogenase (S-LDH; P less than .005), serum beta 2 microglobulin (S -beta 2m; P less than .01), and serum calcium (S-Ca; P less than .025) and inversely correlated with haemoglobin (P less than .025). Our results indicate that 1) radioimmunoassay is suitable for the measurement of human IL-6 in serum; 2) high S-IL-6 levels are observed in a small number of patients with MG; and 3) S-IL-6 level correlates with tumour cell mass in patients with overt MM.
白细胞介素 -6（IL -6）被证明是人类浆细胞瘤细胞强大的自分泌或旁分泌生长因子。通过生物测定法发现，在浆细胞发育异常中，高血清IL -6（S -IL -6）水平与疾病严重程度相关。由于其他细胞因子可能干扰生物测定，我们开发了一种特异性放射免疫测定法，以研究102例单克隆丙种球蛋白病（MG）患者的S -IL -6水平。使用兔多克隆抗IL -6抗体和重组人IL -6作为标准品，通过双抗体放射免疫测定法研究S -IL -6水平。标准品与零有显著差异的最低值为78 pg/ml。批内和批间精密度的平均变异系数分别为3.72%和5.5%。平均分析回收率为113%，通过稀释试验显示IL -6标准品与S -IL -6具有免疫化学同一性。在所有检测血清中均检测到IL -6。检测了66名健康志愿者以及43例急性白血病或恶性淋巴瘤患者的血清作为对照。健康受试者的S -IL -6值为294±86 pg/ml。MG分为多发性骨髓瘤（MM）、巨球蛋白血症和意义未明的单克隆丙种球蛋白病（MGUS）。MG患者的S -IL -6水平分布显著高于健康受试者，但低于急性白血病或霍奇金淋巴瘤患者。对55例MM患者的结果与其他生物学参数进行了关联分析。S -IL -6水平与骨髓浆细胞增多（P＜0.0005）、血清乳酸脱氢酶（S -LDH；P＜0.005）、血清β2微球蛋白（S -β2m；P＜0.01）和血清钙（S -Ca；P＜0.025）相关，与血红蛋白呈负相关（P＜0.025）。我们的结果表明：1）放射免疫测定法适用于测定血清中的人IL -6；2）少数MG患者观察到高S -IL -6水平；3）在明显MM患者中，S -IL -6水平与肿瘤细胞数量相关。
