Shin Hye-Young, Shin Tae-Yong, Seo Sang-Wan, An Hyo-Jin, Kwon Yong-Taek, Song Bong-Keun, Lee Eon-Jeong, Kim Sung-Hoon, Kim Yun-Kyung, Hong Seung-Heon, Kim Hyung-Min
Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, Dongdaemun-Gu, Seoul, Korea.
Biol Pharm Bull. 2004 Oct;27(10):1521-6. doi: 10.1248/bpb.27.1521.
WooKiEum (WKE) has been used for the purpose of the development of increased immune-system strength in Korea. In the present study, we examined the anti-immobility effect of WKE on the forced swimming test (FST), and then measured blood biochemical parameters related to fatigue: glucose (Glc), blood urea nitrogen (BUN), lactic dehydrogenase (LDH), creatinine, and total protein (TP). WKE (0.1, 1 g/kg) was administered orally to mice for 7 d. After 2 d, the immobility time was decreased in the WKE-administered group. After 7 d, the immobility time was significantly decreased in the WKE-administered group (64.6+/-9.0 s for 0.1 g/kg) in comparison with the control group (101.3+/-32.7 s). In addition, amount of Glc in the blood serum was increased, whereas the contents of BUN, LDH and TP decreased in the WKE-administered group. Next, we investigated the effect of WKE on the production of cytokines in a human T-cell line, MOLT-4 cells and mouse peritoneal macrophages. WKE (1 mg/ml) significantly increased interferon (IFN)-gamma and TNF-alpha production compared with the media control (about 2.2-fold for IFN-gamma, about 1.7-fold for TNF-alpha, p<0.05) after 24 h. WKE increased the protein expression of IFN-gamma in MOLT-4 cells. These results suggest that WKE may be useful in immune function improvement.
在韩国，宇气银（WKE）已被用于增强免疫系统的目的。在本研究中，我们检测了WKE在强迫游泳试验（FST）中的抗不动效应，然后测量了与疲劳相关的血液生化参数：葡萄糖（Glc）、血尿素氮（BUN）、乳酸脱氢酶（LDH）、肌酐和总蛋白（TP）。将WKE（0.1、1 g/kg）口服给予小鼠，持续7天。2天后，给予WKE的组的不动时间减少。7天后，与对照组（101.3±32.7秒）相比，给予WKE的组（0.1 g/kg时为64.6±9.0秒）的不动时间显著减少。此外，给予WKE的组血清中Glc含量增加，而BUN、LDH和TP的含量降低。接下来，我们研究了WKE对人T细胞系MOLT-4细胞和小鼠腹腔巨噬细胞中细胞因子产生的影响。24小时后，与培养基对照组相比，WKE（1 mg/ml）显著增加了干扰素（IFN）-γ和肿瘤坏死因子（TNF）-α的产生（IFN-γ约为2.2倍，TNF-α约为1.7倍，p<0.05）。WKE增加了MOLT-4细胞中IFN-γ的蛋白表达。这些结果表明，WKE可能有助于改善免疫功能。
