Shin Hye-Young, Jeong Hyun-Ja, Hong Seung-Heon, Um Jae-Young, Shin Tae-Yong, Kwon Soon-Joo, Jee Seon-Young, Seo Bu-Il, Shin Soon-Shik, Yang Deok-Chun, Kim Hyung-Min
College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, Hoegi-Dong, Dongdaemun-Gu, Seoul, South Korea.
Indian J Med Res. 2006 Aug;124(2):199-206.
BACKGROUND & OBJECTIVES: Panax ginseng has been used as a traditional medicine for many years mainly among Asian peoples for developing physical strength. We undertook this study to determine the immune-enhancement effect of P. ginseng using a forced swimming test (FST) and by measuring cytokine production in MOLT-4 cell culture and mouse peritoneal macrophages.
P. ginseng was orally administered to mice once a day for 7 days. The anti-immobility effect of P. ginseng on the FST and blood biochemical parameters related to fatigue, glucose (Glc); blood urea nitrogen (BUN); latic dehydrogenase (LDH); total protein (TP) and production of cytokines in human T cell line, MOLT-4 cells and mouse peritoneal macrophages were investigated.
After two and seven days, the immobility time was decreased in the P. ginsengadministrated mice as compared to the control group; however, this reduction was not significant. In addition, the amount of TP in the blood serum was significantly increased. However, the levels of Glc, BUN, and LDH did not show a significant change. P. ginseng significantly (P<0.05) increased interferon (IFN)-gamma production and expression as compared to control at 48 h in MOLT-4 cells. P. ginseng plus recombinant IFN-gamma instead of P. ginseng alone significantly increased the production of the tumour necrosis factor (TNF)-alpha in the mouse peritoneal macrophages.
INTERPRETATION & CONCLUSION: Our results suggest that P. ginseng may be useful for an immune promoter. Further studies are needed to understand the mechanism of its action.
人参作为一种传统药物，多年来主要在亚洲人群中用于增强体力。我们进行本研究以通过强迫游泳试验（FST）以及检测MOLT-4细胞培养物和小鼠腹腔巨噬细胞中的细胞因子产生情况，来确定人参的免疫增强作用。
给小鼠每日口服人参一次，持续7天。研究人参对FST的抗不动效应以及与疲劳相关的血液生化参数，包括葡萄糖（Glc）、血尿素氮（BUN）、乳酸脱氢酶（LDH）、总蛋白（TP），以及在人T细胞系MOLT-4细胞和小鼠腹腔巨噬细胞中的细胞因子产生情况。
在第2天和第7天，与对照组相比，给予人参的小鼠不动时间有所减少，但这种减少不显著。此外，血清中TP的量显著增加。然而，Glc、BUN和LDH的水平未显示出显著变化。在MOLT-4细胞中，与人参单独处理相比，人参在48小时时显著（P<0.05）增加了干扰素（IFN）-γ的产生和表达。人参加重组IFN-γ而非单独的人参显著增加了小鼠腹腔巨噬细胞中肿瘤坏死因子（TNF）-α的产生。
我们的结果表明人参可能作为一种免疫促进剂有用。需要进一步研究以了解其作用机制。
