Motai Tsunetake, Kitanaka Susumu
College of Pharmacy, Nihon University, Chiba, Japan.
Chem Pharm Bull (Tokyo). 2004 Oct;52(10):1215-8. doi: 10.1248/cpb.52.1215.
Four new sesquiterpene coumarin derivatives, fukanemarin B (1), fukanefuromarin E (2), fukanefuromarin F (3) and fukanefuromarin G (5) were isolated from a 80% aqueous methanol extract of the roots of Ferula fukanensis. The structures were elucidated based on spectral evidence, especially heteronuclear multiple-bond connectivity (HMBC) and high-resolution MS. The 80% aqueous methanol extract of the roots of Ferula fukanensis (FFE) and the sesquiterpene coumarin derivatives inhibited nitric oxide (NO) production and inducible NO synthase (iNOS) gene expression by a murine macrophage-like cell line (RAW 264.7), which was activated by lipopolysaccharide (LPS) and recombinant mouse interferon-gamma (IFN-gamma).
从福海阿魏（Ferula fukanensis）根的80%甲醇水溶液提取物中分离得到了4个新的倍半萜香豆素衍生物，即福海阿魏素B（1）、福海阿魏呋喃素E（2）、福海阿魏呋喃素F（3）和福海阿魏呋喃素G（5）。基于光谱证据，尤其是异核多键连接（HMBC）和高分辨率质谱对其结构进行了鉴定。福海阿魏（Ferula fukanensis）根的80%甲醇水溶液提取物（FFE）和倍半萜香豆素衍生物可抑制由脂多糖（LPS）和重组小鼠干扰素-γ（IFN-γ）激活的小鼠巨噬细胞样细胞系（RAW 264.7）中一氧化氮（NO）的产生以及诱导型NO合酶（iNOS）基因的表达。
