从新疆阿魏中分离具有神经抗炎活性的倍半萜香豆素：结构导向方法。
Structure-guided isolation of anti-neuroinflammatory sesquiterpene coumarins from Ferula sinkiangensis.
机构信息
School of Traditional Chinese Materia Medica, Key Laboratory of Innovative Traditional Chinese Medicine for Major Chronic Diseases of Liaoning Province, Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City, Shenyang Pharmaceutical University, Shenyang 110016, China.
College of Life and Health Sciences, Northeastern University, Shenyang 110004, China.
出版信息
Chin J Nat Med. 2024 Jul;22(7):643-653. doi: 10.1016/S1875-5364(24)60619-9.
The resin of Ferula sinkiangensis has been traditionally utilized for treating gastrointestinal disorders, inflammation, tumors, various cancers, and alopecia areata. The primary bioactive constituents, sesquiterpene coumarins, have demonstrated notable therapeutic potential against neuroinflammation. In this study, a structure-guided fractionation method was used to isolate nine novel sesquiterpene coumarins from the resin of F. sinkiangensis. These compounds were characterized and structurally elucidated using comprehensive physicochemical and spectroscopic techniques, including calculated electronic circular dichroism (ECD). Anti-neuroinflammatory assays revealed that compounds 2, 3, and 6 significantly inhibited nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated BV2 microglial cells, with IC values ranging from 1.63 to 12.25 μmol·L.
新疆阿魏树脂在传统上被用于治疗胃肠道疾病、炎症、肿瘤、各种癌症和斑秃。其主要的生物活性成分——倍半萜香豆素，已被证明对神经炎症具有显著的治疗潜力。在这项研究中，采用基于结构的分段方法，从新疆阿魏树脂中分离出 9 种新型倍半萜香豆素。这些化合物通过全面的物理化学和光谱技术进行了鉴定和结构解析，包括计算电子圆二色性（ECD）。抗神经炎症试验表明，化合物 2、3 和 6 显著抑制脂多糖（LPS）刺激的 BV2 小胶质细胞中一氧化氮（NO）的产生，IC 值范围为 1.63 到 12.25 μmol·L。
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