Ledda-Columbano G M, Coni P, Faa G, Manenti G, Columbano A
Istituto di Patologia Sperimentale, Università di Cagliari, Italy.
Am J Pathol. 1992 Mar;140(3):545-9.
A single administration of the inhibitor of protein synthesis cycloheximide results in the occurrence of apoptosis in rat liver. The presence of intracellular apoptotic bodies was detected as early as 2 hours after treatment. No evidence of cell necrosis could be observed by histologic and biochemical analysis. Apoptosis was followed by an increased expression of testosterone-repressed prostate message-2 RNA, a gene whose activity has been associated to apoptotic cell death in involuting rat prostate. The finding of in vivo induction of apoptosis in nonproliferating cells by an inhibitor of protein synthesis, together with the rapidity and synchrony in the occurrence of cell death make this model potentially useful for the analysis of the kinetics of the apoptotic cycle and in exploring some of the mechanisms of regulation of genes possibly involved in this type of cell death.
单次给予蛋白质合成抑制剂环己酰亚胺会导致大鼠肝脏中细胞凋亡的发生。早在治疗后2小时就检测到细胞内凋亡小体的存在。通过组织学和生化分析未观察到细胞坏死的迹象。细胞凋亡之后，睾酮抑制的前列腺信息-2 RNA的表达增加，该基因的活性与大鼠前列腺退化过程中的凋亡细胞死亡有关。蛋白质合成抑制剂在体内诱导非增殖细胞发生凋亡，以及细胞死亡发生的快速性和同步性，使得该模型对于分析凋亡周期的动力学以及探索可能参与此类细胞死亡的基因调控机制具有潜在的用途。
