Rambourg A, Clermont Y, Chrétien M, Olivier L
Département de biologie cellulaire et moléculaire du CEA, Centre d'études nucléaires de Saclay, Gif-sur-Yvette, France.
Anat Rec. 1992 Feb;232(2):169-79. doi: 10.1002/ar.1092320202.
The mode of secretory granule formation in prolactin cells was analyzed in thin or thick sections of pituitary glands from non-lactating or lactating female as well as from male rats. In all these animals, the Golgi apparatus of prolacting cells consists of a continuous twisted ribbon-like structure that branches and anastomoses to form a hollow sphere located in the juxtanuclear area. The early signs of secretory granule formation are observed along the trans-aspect of the Golgi ribbon where progranules appear as focal distensions simultaneously occurring anywhere in the last trans thiamine pyrophosphatase (TPPase)-containing Golgi element. In the transmost Golgi saccule, such dilatations usually contain several nodular masses of electron opaque material which are separated from each other and from the saccular membrane by a less intensely stained material. While this transmost saccule becomes more fenestrated, its focal polynodular distensions seemingly yield polynodular tubular progranules which are initially closely apposed and usually parallel to the trans face of the Golgi ribbon. Subsequently, these progranules, which frequently show small membranous tubules or tubular networks attached to them, are seen some distance from the Golgi stacks and progressively transform into the more compact polymorphous granules characteristic of prolactin cells. These observations suggest that the polynodular tubular progranules arise by fragmentation of portions of the trans-Golgi elements rather than by fusion of small uninodular granules budding from the edges of a trans-Golgi saccule. Once the progranules have been liberated, the rest of the transmost Golgi element appears to break down into small residual networks, tubules, and vesicles. Thus, in prolactin cells as in other glandular cells, the whole transmost Golgi element would fragment during formation of prosecretory granules.
对非泌乳或泌乳雌性以及雄性大鼠垂体的薄切片或厚切片中催乳素细胞分泌颗粒的形成模式进行了分析。在所有这些动物中，催乳素细胞的高尔基体由连续的扭曲带状结构组成，该结构分支并吻合形成位于近核区域的中空球体。分泌颗粒形成的早期迹象沿着高尔基体带的反面观察到，前颗粒表现为局灶性扩张，同时出现在最后一个含焦磷酸硫胺素酶（TPPase）的高尔基体元件的任何位置。在最反面的高尔基体囊泡中，这种扩张通常包含几个电子不透明物质的结节状团块，它们彼此之间以及与囊泡膜之间被染色较浅的物质分隔开。当这个最反面的囊泡变得更具窗孔状时，其局灶性多结节扩张似乎产生多结节管状前颗粒，这些前颗粒最初紧密相邻且通常与高尔基体带的反面平行。随后，这些经常显示附着有小膜性小管或管状网络的前颗粒，在离高尔基体堆叠一定距离处可见，并逐渐转变为催乳素细胞特有的更致密的多形性颗粒。这些观察结果表明，多结节管状前颗粒是由反式高尔基体元件部分的碎片化产生的，而不是由从反式高尔基体囊泡边缘出芽的小单结节颗粒融合产生的。一旦前颗粒被释放，最反面的高尔基体元件的其余部分似乎分解成小的残余网络、小管和囊泡。因此，在催乳素细胞中，如同在其他腺细胞中一样，整个最反面的高尔基体元件在分泌前颗粒形成过程中会发生碎片化。
