Woolstenhulme Mandy T, Jutte Lisa S, Drummond Micah J, Parcell Allen C
Human Performance Research Center, 120-E Richards Building, Brigham Young University, Provo, Utah 84602, USA.
Muscle Nerve. 2005 Jan;31(1):20-4. doi: 10.1002/mus.20170.
To investigate the role desmin may play in muscular adaptation to exercise, we measured desmin protein content in the vastus lateralis muscle of seven untrained men in response to 8 weeks of high-intensity cycle training. Training involved 15-s sprints separated by rest for 5 min. Subjects began with four sprints twice per week, and progressed to six sprints three times per week. Peak power was measured before and after training with a 30-s maximal sprint test. Mean power during the first 15 s increased significantly after training (P < 0.05). Desmin and actin protein levels were determined by immunoblotting, from pretraining and posttraining muscle biopsies. Desmin protein levels were increased by 60% after training (P < 0.01), whereas actin protein levels did not change with training. We conclude that the cytoskeletal protein desmin increases in response to a high-tension, concentric-only load consequent to sprint training. Desmin appears to increase as the force generating capacity of the muscle increases. A reinforced desmin cytoskeleton may be necessary for increased force generation by the muscle.
为了研究结蛋白在肌肉对运动的适应性中可能发挥的作用，我们测量了7名未经训练的男性在进行8周高强度自行车训练后，其股外侧肌中的结蛋白含量。训练包括15秒的冲刺，中间休息5分钟。受试者开始时每周进行两次，每次4次冲刺，之后逐渐增加到每周3次，每次6次冲刺。在训练前后通过30秒最大冲刺测试测量峰值功率。训练后前15秒的平均功率显著增加（P < 0.05）。通过免疫印迹法测定训练前和训练后肌肉活检组织中的结蛋白和肌动蛋白水平。训练后结蛋白水平增加了60%（P < 0.01），而肌动蛋白水平并未随训练而改变。我们得出结论，细胞骨架蛋白结蛋白会因冲刺训练产生的高张力、仅向心负荷而增加。随着肌肉产生力量的能力增加，结蛋白似乎也会增加。增强的结蛋白细胞骨架对于肌肉增加力量产生可能是必要的。
