Parcell Allen C, Woolstenhulme Mandy T, Sawyer Robert D
Human Performance Research Center, Brigham Young University, Provo, Utah, USA.
J Strength Cond Res. 2009 Mar;23(2):359-65. doi: 10.1519/JSC.0b013e318198fd62.
The muscle cytoskeleton is necessary for the effective transmission of forces generated by actin-myosin interactions. We have examined the impact of muscle force and exercise volume on the cytoskeleton by measuring desmin and dystrophin content in human skeletal muscle after 12 weeks of progressive resistance or endurance cycle training. Muscle biopsies of the vastus lateralis were obtained before and after training. Desmin and dystrophin content was determined using immunoblotting techniques. After resistance training, desmin content increased 82 +/- 18% (p < 0.05), whereas there was no change in desmin content with endurance cycling. Dystrophin content did not change in either group. One-repetition maximum and VO2max increased (p < 0.05) in the resistance and endurance groups, respectively. These data demonstrate that a high-tension stimulus impacts the cytoskeleton in contrast to high-volume concentric contractions. The tensile loading and eccentric components of resistance training are implicated in desmin alterations. Indeed, the functional improvements resulting from resistance training may be related in part to the mechanical integration provided by the desmin protein.
肌细胞骨架对于肌动蛋白-肌球蛋白相互作用所产生力量的有效传递是必需的。我们通过测量进行12周渐进性抗阻或耐力循环训练后人体骨骼肌中结蛋白和肌营养不良蛋白的含量，研究了肌肉力量和运动量对细胞骨架的影响。在训练前后获取股外侧肌的肌肉活检样本。使用免疫印迹技术测定结蛋白和肌营养不良蛋白的含量。抗阻训练后，结蛋白含量增加了82±18%（p<0.05），而耐力骑行训练后结蛋白含量没有变化。两组中肌营养不良蛋白含量均未改变。抗阻组和耐力组的一次重复最大负荷和最大摄氧量分别增加（p<0.05）。这些数据表明，与大量的向心收缩相比，高张力刺激会影响细胞骨架。抗阻训练的拉伸负荷和离心成分与结蛋白的改变有关。事实上，抗阻训练带来的功能改善可能部分与结蛋白提供的机械整合有关。
