Houzé P, Beltz S, Samba C, Malvy D, Bousquet B, Gourmel B
Laboratoire de biochimie A, Hôpital Saint Louis, Paris.
Ann Biol Clin (Paris). 2004 Sep-Oct;62(5):539-46.
In tropical countries. vitamin A deficiency is one of the most important dietary deficiencies. Its monitoring usually involves analysis of retinol after venipuncture with some difficulties (disease transmission, religious belief). Sample collection on Dried Blood Spot (DBS) is less invasive and safer. Sample storage is easier. We developed a liquid chromatography method with electrochemical detection to measure DBS retinol. Retinol acetate was used as an internal standard. The method is linear up to 2.5 microM with a detection limit of 0.04 microM. Precision is below 10% and DBS retinol recovery overage is 90%. DBS retinol concentration decreased during 7 days after sampling, it is necessary to wait this delay before to determine vitamin A concentrations. In Congolese children DBS retinol measurement showed a severe vitamin A deficiency in 8% of them. This percentage is closely correlated with clinical parameters.
在热带国家，维生素A缺乏是最重要的膳食缺乏症之一。其监测通常涉及静脉穿刺后对视黄醇进行分析，但存在一些困难（疾病传播、宗教信仰）。干血斑（DBS）样本采集侵入性较小且更安全。样本储存更容易。我们开发了一种带有电化学检测的液相色谱法来测定DBS中的视黄醇。醋酸视黄酯用作内标。该方法在高达2.5微摩尔时呈线性，检测限为0.04微摩尔。精密度低于10%，DBS视黄醇回收率超过90%。采样后7天内DBS视黄醇浓度会下降，在测定维生素A浓度之前有必要等待这段时间。在刚果儿童中，DBS视黄醇测量显示8%的儿童存在严重维生素A缺乏。这一百分比与临床参数密切相关。
