Stunzenas Virmantas, Cryan Jason R, Molloy Daniel P
Institute of Ecology, Vilnius University, Akademijos 2, Vilnius LT 2600, Lithuania.
Parasitol Int. 2004 Sep;53(3):223-8. doi: 10.1016/j.parint.2003.12.003.
The most frequently used antimitotic agent in cytogenetic studies is colchicine. We investigated whether the initial treatment of trematodes for karyological analysis with colchicine would have mutagenic or degradational effect on rDNA sequences. Dreissena polymorpha is the intermediate host of Phyllodistomum folium and Bucephalus polymorphus, and the sporocyst stage of these trematode species develop, respectively, in the gills and gonads of this mussel. Sporocysts of P. folium and B. polymorphus were obtained from D. polymorpha collected from waterbodies in Belarus and in Lithuania. 5.8S and 28S rDNA genes, ITS1 and ITS2 of P folium and B. polymorphus were sequenced and compared, and no nucleotide sequence differences between colchicine treated and untreated trematodes were found. Based on these results, we conclude that colchicine treatment for 3-5 h has no mutagenic or degradational effect on rDNA sequences. During the course of this investigation, two genetically different P. folium samples were noted in Belarus.
在细胞遗传学研究中最常用的抗有丝分裂剂是秋水仙碱。我们研究了用秋水仙碱对吸虫进行核型分析的初始处理是否会对rDNA序列产生诱变或降解作用。多形饰贝是叶形吸虫和多形牛头吸虫的中间宿主，这些吸虫物种的孢蚴阶段分别在这种贻贝的鳃和性腺中发育。叶形吸虫和多形牛头吸虫的孢蚴取自从白俄罗斯和立陶宛水体采集的多形饰贝。对叶形吸虫和多形牛头吸虫的5.8S和28S rDNA基因、ITS1和ITS2进行了测序和比较，未发现经秋水仙碱处理和未处理的吸虫之间存在核苷酸序列差异。基于这些结果，我们得出结论，3至5小时的秋水仙碱处理对rDNA序列没有诱变或降解作用。在这项研究过程中，在白俄罗斯注意到两个遗传上不同的叶形吸虫样本。
