Tan K K, Aminuddin B S, Tan G H, Sabarul Afian M, Ng M H Angela, Fauziah O, Ruszymah B H I
Department of Orthopaedics & Traumatology, Faculty of Medicine, Universiti Kebangsaan Malaysia.
Med J Malaysia. 2004 May;59 Suppl B:43-4.
The strategy used to generate tissue-engineered bone construct, in view of future clinical application is presented here. Osteoprogenitor cells from periosteum of consenting scoliosis patients were isolated. Growth factors viz TGF-B2, bFGF and IGF-1 were used in concert to increase cell proliferation during in vitro cell expansion. Porous tricalcium phosphate (TCP)-hydroxyapatite (HA) scaffold was used as the scaffold to form 3D bone construct. We found that the addition of growth factors, greatly increased cell growth by 2 to 7 fold. TCP/HA proved to be the ideal scaffold for cell attachment and proliferation. Hence, this model will be further carried out on animal trial.
本文介绍了鉴于未来临床应用而用于生成组织工程骨构建体的策略。从同意参与的脊柱侧弯患者的骨膜中分离出骨祖细胞。在体外细胞扩增过程中，协同使用生长因子转化生长因子-β2（TGF-B2）、碱性成纤维细胞生长因子（bFGF）和胰岛素样生长因子-1（IGF-1）来增加细胞增殖。使用多孔磷酸三钙（TCP）-羟基磷灰石（HA）支架作为构建三维骨构建体的支架。我们发现添加生长因子可使细胞生长大幅增加2至7倍。TCP/HA被证明是细胞附着和增殖的理想支架。因此，该模型将进一步进行动物试验。
