Zhu Shi-Jiang, Choi Byung-Ho, Huh Jin-Young, Jung Jae-Hyung, Kim Byung-Yong, Lee Seoung-Ho
Department of Oral & Maxillofacial Surgery, College of Dentistry, Yonsei University, Seoul, South Korea.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Feb;101(2):164-9. doi: 10.1016/j.tripleo.2005.04.006. Epub 2005 Sep 23.
For tissue-engineered bone formation, autogenous osteogenic cells are of paramount importance for successful bone formation. In order to investigate the donor cell-related differences in tissue-engineered bone, cultured bone marrow mesenchymal stem cells, cultured alveolar bone cells, and cultured periosteal cells were examined for their in vivo potential to form bone. These cells were isolated from dogs, expanded in vitro, mixed with autologous fibrin glue and BMP-2, and then injected into the subcutaneous space on the dorsum of nude mice. Bone formation was evaluated at 12 weeks. Histomorphometric analysis demonstrated that the subcutaneous nodules formed in nude mice contained 26.9% newly formed bone when using the bone marrow mesenchymal stem cells, 41.1% newly formed bone when using the alveolar bone cells, and 58.2% newly formed bone when using the periosteal cells. The results suggest that periosteal cells are the best choice for enhancing bone formation in tissue engineering of bone regeneration.
对于组织工程骨的形成，自体成骨细胞对于成功的骨形成至关重要。为了研究组织工程骨中供体细胞相关的差异，检测了培养的骨髓间充质干细胞、培养的牙槽骨细胞和培养的骨膜细胞在体内形成骨的潜力。这些细胞从犬体内分离，在体外扩增，与自体纤维蛋白胶和BMP-2混合，然后注射到裸鼠背部的皮下间隙。在12周时评估骨形成情况。组织形态计量学分析表明，使用骨髓间充质干细胞时，裸鼠皮下结节中新生骨占26.9%；使用牙槽骨细胞时，新生骨占41.1%；使用骨膜细胞时，新生骨占58.2%。结果表明，骨膜细胞是增强骨再生组织工程中骨形成的最佳选择。
