Song Jian-Min, Li Bao-Yun, You Ming-Shan, Liang Rong-Qi, Chang Cheng, Liu Shou-Bin, Tang Zhao-Hui, Liu Guang-Tian
Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100094, China.
Yi Chuan Xue Bao. 2004 Jan;31(1):81-6.
Fourteen wheat cultivars were identified into six types of Wx proteins combinations using 6% SDS-PAGE. PCR primers were designed according to the three Wx genes sequences and their mutants, respectively. A 327 bp-band was amplified from the Wx-A1 mutant,while the band was absent for the normal alleles at the Wx-A1 locus,as well as the presence or absence of a 187 bp PCR fragment at the Wx-B1 locus and a 700 bp PCR fragment at the Wx-D1 locus, respectively, corresponding to the normal and mutant alleles. Compared with the former studies, shorter and more different PCR products at three loci, amplified by the primers designed for Wx-B1 gene can be separated in 2% agarose gel, which enables screening breeding lines for noodle use faster and effectively.
