Lu Long-Dou, Hou Cai-Ling, Chen Long, Yin Gui-Hong, Deng Chuan-Liang, Gao Wu-Jun, Yang Xu-Qin, Tan Guang-Xuan
College of Life Science, Henan Normal University, Xinxiang 453007, China.
Yi Chuan. 2009 Aug;31(8):844-8. doi: 10.3724/sp.j.1005.2009.00844.
Multiple-PCR was conducted to establish a stable PCR system for identifying the three Wx genes in wheat. Two pairs of primers were employed to amplify Wx-A1, Wx-B1, and Wx-D1 genes of wheat, with the target sequences of 230 bp/265 bp, 854 bp, and 204 bp, respectively. The results showed that Wx-A1, Wx-B1, and Wx-D1 can be detected simultaneously in a single reaction. This method proved to be repeatable and low cost for evaluation of wheat quality properties in breeding program. This multiple-PCR technique can be efficiently used in marker-assisted selection for Wx genes, which will improve selection procedure for waxy wheat.
进行多重聚合酶链式反应（Multiple-PCR）以建立一个稳定的用于鉴定小麦中三个蜡质（Wx）基因的聚合酶链式反应（PCR）系统。使用两对引物来扩增小麦的Wx-A1、Wx-B1和Wx-D1基因，其目标序列分别为230碱基对/265碱基对、854碱基对和204碱基对。结果表明，Wx-A1、Wx-B1和Wx-D1可以在单个反应中同时被检测到。该方法被证明在育种计划中评估小麦品质特性方面具有可重复性且成本低。这种多重聚合酶链式反应（Multiple-PCR）技术可有效地用于Wx基因的分子标记辅助选择，这将改善糯小麦的选择程序。
