Ishikawa Fusako, Oishi Mitsuo, Kimura Keisuke, Yasui Akiko, Saito Kazuo
Tokyo Metropolitan Institute of Public Health, 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan.
Shokuhin Eiseigaku Zasshi. 2004 Jun;45(3):150-5. doi: 10.3358/shokueishi.45.150.
A method for the determination of 12 synthetic food dyes (Amaranth, Erythrosine, Allura Red AC, New Coccine, Phloxine, Rose Bengal, Acid Red, Tartrazine, Sunset Yellow FCF, Fast Green FCF, Brilliant Blue FCF, Indigo Carmine) in food was developed using capillary electrophoresis (CE) with photodiode array detection. The dyes were extracted with water and 0.5% ammonia-ethanol (1:1) mixture, and cleaned up using solid-phase extraction (Sep-Pak Plus tC18). The dyes were eluted with methanol from the cartridge. The dyes were separated by CE on a bubble cell fused-silica capillary (72 cm to the detector, 75 microm i.d.) using 20% acetonitrile in a mixture of 10 mmol/L potassium phosphate, monobasic and 5 mmol/L sodium carbonate (pH 10.0) as the running buffer. Identifications of the dyes were performed on the basis of the migration time and the absorbance spectrum of each peak. The coefficients of variation of the migration times and the peak areas were 0.28-0.62% and 1.84-4.30%, respectively (n = 5). The identification limits using the absorbance spectra of the dyes were 10 microg/mL for Brilliant Blue FCF and Fast Green FCF, and 5 microg/mL for the other 10 dyes. The recoveries of the 12 dyes from pickles, soft drinks and candies at the level of 10 microg/g were 70.0-101.5%. The method was applied to the analysis of dyes in foods. The dyes detected by CE were in agreement with those detected by paper chromatography.
建立了一种利用毛细管电泳（CE）结合光电二极管阵列检测法测定食品中12种合成食用色素（苋菜红、赤藓红、诱惑红、新红、福禄考猩红、玫瑰红、酸性红、柠檬黄、日落黄FCF、亮绿FCF、亮蓝FCF、靛蓝胭脂红）的方法。色素用水和0.5%氨 - 乙醇（1:1）混合液提取，并用固相萃取（Sep - Pak Plus tC18）进行净化。色素用甲醇从柱中洗脱。在气泡池熔融石英毛细管（至检测器72 cm，内径75μm）上，以10 mmol/L磷酸二氢钾和5 mmol/L碳酸钠（pH 10.0）的混合液中含20%乙腈作为运行缓冲液，通过CE对色素进行分离。根据各峰的迁移时间和吸收光谱对色素进行鉴定。迁移时间和峰面积的变异系数分别为0.28 - 0.62%和1.84 - 4.30%（n = 5）。利用色素吸收光谱的鉴定限，亮蓝FCF和亮绿FCF为10μg/mL，其他10种色素为5μg/mL。在10μg/g水平下，12种色素在泡菜、软饮料和糖果中的回收率为70.0 - 101.5%。该方法应用于食品中色素的分析。CE检测到的色素与纸色谱检测结果一致。
