Pérez-Urquiza M, Beltrán J L
Departament de Química Analítica, Universitat de Barcelona, Spain.
J Chromatogr A. 2000 Nov 17;898(2):271-5. doi: 10.1016/s0021-9673(00)00841-4.
A rapid method based on capillary zone electrophoresis coupled with photodiode-array detection has been developed to determine the dyes Tartrazine E-102, Sunset Yellow FCF E110, Amaranth E-123, New Coccine E-124, Patent Blue V calcium salt E-131 and Allura Red AC E-129 in foodstuffs. Separation was done by using a Bare CElect-FS75 CE column, using a 10 mM phosphate buffer at pH 11.0. Hydrodynamic injections at 0.5 p.s.i. for 4 s (21 nl of sample) and 20 kV separation voltage were used. The quantitation limits for the six dyes ranged from 3 to 6 microg/ml. A linear relationship between 3 to 95 microg/ml, with correlation coefficient better than 0.995 was obtained. This method has been applied to the determination of the studied dyes in beverages, jellies and syrups.
已开发出一种基于毛细管区带电泳结合光电二极管阵列检测的快速方法，用于测定食品中的柠檬黄E-102、日落黄FCF E110、苋菜红E-123、新红E-124、专利蓝V钙盐E-131和诱惑红AC E-129。使用Bare CElect-FS75 CE柱，以pH 11.0的10 mM磷酸盐缓冲液进行分离。采用0.5 p.s.i.的流体动力学进样4 s（21 nl样品）和20 kV的分离电压。六种染料的定量限为3至6 μg/ml。在3至95 μg/ml之间获得了线性关系，相关系数优于0.995。该方法已应用于饮料、果冻和糖浆中所研究染料的测定。
