Suzuki S, Shirao M, Aizawa M, Nakazawa H, Sasa K, Sasagawa H
National Institute of Public Health, Tokyo, Japan.
J Chromatogr A. 1994 Oct 7;680(2):541-7. doi: 10.1016/0021-9673(94)85153-0.
A method for the determination of synthetic tar dyes used as food additives using capillary electrophoresis with photodiode-array detection was investigated. The dyes Erythrosine (R-3), Phloxine (R-104), Rose Bengal (R-105), Acid Red (R-106), Amaranth (R-2), New Coccine (R-102) and Allura Red AC (R-40) were separated on a capillary column (50 cm x 75 microns I.D.) and identified from the absorbance spectra of each peak. The electrophoresis buffer used was a mixture of 25 mM sodium phosphate buffer and 25 mM sodium borate buffer (1:1) (pH 8.0) containing 10 mM sodium dodecyl sulfate (SDS). Substitution of beta-cyclodextrin for SDS in the electrolyte buffer was effective for the separation of R-2 and R-102. This modified method could be employed as an additional assay method for these two dyes.
研究了一种使用带光电二极管阵列检测的毛细管电泳法测定用作食品添加剂的合成焦油染料的方法。在毛细管柱（50 cm×75微米内径）上分离了赤藓红（R - 3）、焰红染料（R - 104）、孟加拉玫瑰红（R - 105）、酸性红（R - 106）、苋菜红（R - 2）、新红（R - 102）和诱惑红（R - 40）等染料，并根据每个峰的吸收光谱进行鉴定。所用的电泳缓冲液是25 mM磷酸钠缓冲液和25 mM硼酸钠缓冲液（1:1）（pH 8.0）的混合物，其中含有10 mM十二烷基硫酸钠（SDS）。在电解质缓冲液中用β - 环糊精替代SDS对R - 2和R - 102的分离有效。这种改进的方法可作为这两种染料的一种补充检测方法。
