内源性反式作用小干扰RNA调控拟南芥信使核糖核酸的积累。

Endogenous trans-acting siRNAs regulate the accumulation of Arabidopsis mRNAs.

作者信息

Vazquez Franck, Vaucheret Hervé, Rajagopalan Ramya, Lepers Christelle, Gasciolli Virginie, Mallory Allison C, Hilbert Jean-Louis, Bartel David P, Crété Patrice

机构信息

Laboratoire de physiologie de la différenciation végétale, UPRES-EA3569, IFR118, EPT1016, Université des Sciences et Technologies de Lille, Bât. SN2, cité scientifique, 59650 Villeneuve d'Ascq Cedex, France.

出版信息

Mol Cell. 2004 Oct 8;16(1):69-79. doi: 10.1016/j.molcel.2004.09.028.

DOI:10.1016/j.molcel.2004.09.028

PMID:15469823

Abstract

Here we describe a set of endogenous short interfering RNAs (siRNAs) in Arabidopsis, some of which direct the cleavage of endogenous mRNAs. These siRNAs correspond to both sense and antisense strands of a noncoding RNA (At2g27400) that apparently is converted to double-stranded RNA and then processed in 21 nt increments. These siRNAs differ from previously described regulatory small RNAs in two respects. First, they require components of the cosuppression pathway (RDR6 and SGS3) and also components of the microRNA (miRNA) pathway (AGO1, DCL1, HEN1, and HYL1) but not components needed for heterochromatic siRNAs (DCL3 and RDR2), another class of endogenous plant siRNAs. Second, these siRNAs repress the expression of genes that have little overall resemblance to the genes from which they originate, a characteristic previously reported only for miRNAs. The identification of this silencing pathway provides yet another dimension to posttranscriptional mRNA regulation in plants.

摘要

我们在此描述了拟南芥中的一组内源性小干扰RNA（siRNA），其中一些可指导内源性mRNA的切割。这些siRNA对应于一种非编码RNA（At2g27400）的正义链和反义链，该非编码RNA显然会转化为双链RNA，然后以21个核苷酸的增量进行加工。这些siRNA在两个方面与先前描述的调控性小RNA不同。第一，它们需要共抑制途径的组分（RDR6和SGS3）以及微小RNA（miRNA）途径的组分（AGO1、DCL1、HEN1和HYL1），但不需要异染色质siRNA（DCL3和RDR2）所需的组分，异染色质siRNA是另一类内源性植物siRNA。第二，这些siRNA抑制的基因与它们起源的基因总体上几乎没有相似性，这一特征以前仅在miRNA中报道过。这种沉默途径的鉴定为植物转录后mRNA调控提供了另一个层面。