Supp Dorothy M, Karpinski Andrea C, Boyce Steven T
Research Department, Shriners Hospitals for Children, Cincinnati Burns Hospital, 3229 Burnet Avenue, Cincinnati, OH 45229, USA.
Burns. 2004 Nov;30(7):643-8. doi: 10.1016/j.burns.2004.03.012.
Defensins are effector molecules of the innate host defense system with antimicrobial activity against a variety of pathogens, including microorganisms commonly found in burn units. beta-Defensins are variably expressed in the epithelia of skin and other organs. Cultured skin substitutes (CSS) grafted to burn wounds lack a vascular plexus and are therefore more susceptible to microbial contamination than split thickness skin autograft. To investigate whether beta-defensins can contribute to host defense in CSS, we examined expression of human beta-defensins HBD-1, HBD-2, and HBD-3 in cultured keratinocytes and CSS from uninjured donors and burn patients. HBD-1 was expressed in all keratinocyte strains analyzed. HBD-2 expression in keratinocyte monolayers was highly variable but did not correlate with burn injury. HBD-3 was expressed at variable levels in all but one keratinocyte strain. CSS were prepared from two donors that lacked expression of HBD-2 in keratinocyte monolayers. All three genes were readily detected in CSS from both donors, suggesting up-regulation of HBD-2 and HBD-3. In sections of CSS, HBD-1, HBD-2, and HBD-3 proteins were localized to distinct epidermal regions. We conclude that beta-defensins can potentially contribute to innate immunity in CSS, but their levels may be too low to prevent contamination after grafting.
防御素是天然宿主防御系统的效应分子，对多种病原体具有抗菌活性，包括烧伤病房常见的微生物。β-防御素在皮肤和其他器官的上皮细胞中表达各异。移植到烧伤创面的培养皮肤替代物（CSS）缺乏血管丛，因此比中厚皮片自体移植更容易受到微生物污染。为了研究β-防御素是否有助于CSS中的宿主防御，我们检测了来自未受伤供体和烧伤患者的培养角质形成细胞及CSS中人类β-防御素HBD-1、HBD-2和HBD-3的表达。在所分析的所有角质形成细胞株中均表达HBD-1。角质形成细胞单层中HBD-2的表达高度可变，但与烧伤损伤无关。除一个角质形成细胞株外，所有角质形成细胞株中HBD-3均以可变水平表达。从角质形成细胞单层中缺乏HBD-2表达的两名供体制备CSS。在两名供体的CSS中均能轻易检测到所有三个基因，提示HBD-2和HBD-3上调。在CSS切片中，HBD-1、HBD-2和HBD-3蛋白定位于不同的表皮区域。我们得出结论，β-防御素可能有助于CSS中的固有免疫，但其水平可能过低，无法防止移植后污染。
