Faudry Eric, Rocha Priscila S, Vernet Thierry, Lozzi Silene P, Teixeira Antonio R L
Chagas Disease Multidisciplinary Research Laboratory, Faculty of Medicine, University of Brasilia, 70910-900 Brasilia, Brazil.
Insect Biochem Mol Biol. 2004 Oct;34(10):1051-8. doi: 10.1016/j.ibmb.2004.06.016.
Apyrases are nucleoside triphosphate-diphosphohydrolases that remove Pi from ATP and ADP. The blood feeding reduviid Triatoma infestans, which transmits the Trypanosoma cruzi agent of Chagas disease to animals and man, presents in its salivary glands five apyrases with molecular masses of 88, 82, 79, 68 and 67 kDa. These triatomine apyrases have been associated with the prevention of ADP induced platelet aggregation in the host. Here we provide biochemical data showing that these apyrases are stored in the lumen of the salivary gland D1 pairs, and that about one half of the pool of the enzyme is consumed during feeding. After the feeding recovery of apyrases to maximal activity level takes days, thus suggesting de novo protein synthesis. This hypothesis is supported by quantitative RT-PCR analysis which shows an upregulation of the 79 kDa apyrase mRNA level after feeding.
腺苷三磷酸双磷酸酶是一种核苷三磷酸二磷酸水解酶，可从ATP和ADP中去除磷酸根（Pi）。吸血猎蝽（红带锥蝽）可将恰加斯病的病原体克氏锥虫传播给动物和人类，其唾液腺中存在五种分子量分别为88、82、79、68和67 kDa的腺苷三磷酸双磷酸酶。这些锥蝽腺苷三磷酸双磷酸酶与预防宿主中ADP诱导的血小板聚集有关。在此，我们提供的生化数据表明，这些腺苷三磷酸双磷酸酶储存在唾液腺D1对的管腔中，并且在进食过程中约一半的酶库被消耗。进食后，腺苷三磷酸双磷酸酶恢复到最大活性水平需要数天时间，因此提示需要从头进行蛋白质合成。定量RT-PCR分析支持了这一假设，该分析显示进食后79 kDa腺苷三磷酸双磷酸酶的mRNA水平上调。
