Martínez Fernando, Alejandre-Aguilar Ricardo, Moncada Yolanda Hortelano, Espinoza Bertha
Department of Immunology, Biomedical Research Institute, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico.
Am J Trop Med Hyg. 2005 Aug;73(2):321-5.
The Triatiominae (Hemiptera: Reduviidae) are hematophagous hemipters of importance because they transmit Trypanosoma cruzi, the causal agent of Chagas disease. The aim of this study was to define the possible relationships between species of the Phyllosoma complex (Triatoma mazzottii, Triatoma pallidipennis, and Triatoma longipennis) and species of other complexes present in Mexico that have not been previously analyzed (Triatoma lecticularia and Triatoma rubida). In addition, it was determined the inclusion of Triatoma bassolsae in the Phyllosoma complex by using 10 isoenzymatic systems (corresponding to the 14 loci). Results of isoenzymatic study show that between the species of the Phyllosoma complex including Triatoma bassolsae, the polymorphism of the analyzed enzymes ranges from 14% to 50% (P < or = 0.95) and the species from external complexes showed polymorphism values of 43% (Triatoma lecticularia), 43% (Triatoma rubida), and 36% (Triatoma infestans). The genetic tree shows a clear difference between species of the Phyllosoma complex and the other complexes.
锥猎蝽亚科(半翅目:猎蝽科)是重要的吸血半翅目昆虫,因为它们传播恰加斯病的病原体克氏锥虫。本研究的目的是确定叶形复合体的物种(马佐蒂锥猎蝽、淡色锥猎蝽和长翅锥猎蝽)与墨西哥境内其他尚未进行过分析的复合体的物种(床栖锥猎蝽和红锥猎蝽)之间可能存在的关系。此外,通过使用10种同工酶系统(对应于14个基因座)来确定巴氏锥猎蝽是否包含在叶形复合体中。同工酶研究结果表明,在包括巴氏锥猎蝽在内的叶形复合体的物种之间,所分析酶的多态性范围为14%至50%(P≤0.95),而来自外部复合体的物种显示的多态性值分别为43%(床栖锥猎蝽)、43%(红锥猎蝽)和36%(骚扰锥猎蝽)。遗传树显示叶形复合体的物种与其他复合体的物种之间存在明显差异。