Programa Pós-Graduação em Ciências Médicas, Faculty of Medicine, The University of Brasília, Brasília, Brazil.
Programa Pós-Graduação em Ciências Médicas, Faculty of Medicine, The University of Brasília, Brasília, Brazil; Faculty of Ceilândia, The University of Brasília, Brasília, Brazil.
J Proteomics. 2018 Mar 1;174:47-60. doi: 10.1016/j.jprot.2017.12.016. Epub 2017 Dec 27.
Triatoma dimidiata, a Chagas disease vector widely distributed along Central America, has great capability for domestic adaptation as the majority of specimens caught inside human dwellings or in peridomestic areas fed human blood. Exploring the salivary compounds that overcome host haemostatic and immune responses is of great scientific interest. Here, we provide a deeper insight into its salivary gland molecules. We used high-throughput RNA sequencing to examine in depth the T. dimidiata salivary gland transcriptome. From >51 million reads assembled, 92.21% are related to putative secreted proteins. Lipocalin is the most abundant gene family, confirming it is an expanded family in Triatoma genus salivary repertoire. Other putatively secreted members include phosphatases, odorant binding protein, hemolysin, proteases, protease inhibitors, antigen-5 and antimicrobial peptides. This work expands the previous set of functionally annotated sequences from T. dimidiata salivary glands available in NCBI from 388 to 3815. Additionally, we complemented the salivary analysis through proteomics (available data via ProteomeXchange with identifier PXD008510), disclosing the set complexity of 119 secreted proteins and validating the transcriptomic results. Our large-scale approach enriches the pharmacologically active molecules database and improves our knowledge about the complexity of salivary compounds from haematophagous vectors and their biological interactions.
Several haematophagous triatomine species can transmit Trypanosoma cruzi, the etiological agent of Chagas disease. Due to the reemergence of this disease, new drugs for its prevention and treatment are considered priorities. For this reason, the knowledge of vector saliva emerges as relevant biological finding, contributing to the design of different strategies for vector control and disease transmission. Here we report the transcriptomic and proteomic compositions of the salivary glands (sialome) of the reduviid bug Triatoma dimidiata, a relevant Chagas disease vector in Central America. Our results are robust and disclosed unprecedented insights into the notable diversity of its salivary glands content, revealing relevant anti-haemostatic salivary gene families. Our work expands almost ten times the previous set of functionally annotated sequences from T. dimidiata salivary glands available in NCBI. Moreover, using an integrated transcriptomic and proteomic approach, we showed a correlation pattern of transcription and translation processes for the main gene families found, an important contribution to the research of triatomine sialomes. Furthermore, data generated here reinforces the secreted proteins encountered can greatly contribute for haematophagic habit, Trypanosoma cruzi transmission and development of therapeutic agent studies.
分布于中美洲的广泛传播的恰加斯病传播媒介 Triatoma dimidiata 具有很强的适应家庭环境的能力，因为大多数在人类住所内或周围捕获的标本都以人类血液为食。探索克服宿主止血和免疫反应的唾液化合物具有重要的科学意义。在这里，我们深入了解其唾液腺分子。我们使用高通量 RNA 测序深入研究了 T. dimidiata 唾液腺转录组。从组装的超过 5100 万条读取中，92.21%与假定的分泌蛋白有关。脂质运载蛋白是最丰富的基因家族，证实它是 Triatoma 属唾液腺中一个扩展的家族。其他假定的分泌成员包括磷酸酶、气味结合蛋白、溶血素、蛋白酶、蛋白酶抑制剂、抗原 5 和抗菌肽。这项工作将以前在 NCBI 中可获得的 T. dimidiata 唾液腺功能注释序列从 388 个扩展到 3815 个。此外，我们通过蛋白质组学（通过 ProteomeXchange 提供的数据，标识符为 PXD008510）补充了唾液分析，揭示了 119 种分泌蛋白的集合复杂性，并验证了转录组结果。我们的大规模方法丰富了药理学活性分子数据库，并提高了我们对吸血媒介唾液化合物的复杂性及其生物学相互作用的认识。
几种吸血三锥虫物种可以传播恰加斯病的病原体 Trypanosoma cruzi。由于这种疾病的再次出现，预防和治疗这种疾病的新药被认为是优先事项。因此，有关媒介唾液的知识作为相关的生物学发现出现，有助于设计不同的媒介控制和疾病传播策略。在这里，我们报告了中美洲重要的恰加斯病传播媒介 Reduviid 臭虫 Triatoma dimidiata 的唾液腺（唾液组）的转录组和蛋白质组组成。我们的结果是可靠的，并揭示了其唾液腺内容的显著多样性的前所未有的见解，揭示了相关的抗止血唾液基因家族。我们的工作将以前在 NCBI 中可获得的 T. dimidiata 唾液腺的功能注释序列集扩展了近十倍。此外，使用转录组和蛋白质组学的综合方法，我们显示了主要基因家族的转录和翻译过程之间的相关模式，这是对三锥虫唾液组研究的重要贡献。此外，这里生成的数据增强了所遇到的分泌蛋白可以极大地促进吸血习惯、传播 Trypanosoma cruzi 和开发治疗剂的研究。
