Diggle Matthew A, Clarke Stuart C
Scottish Meningococcus and Pneumococcus Reference Laboratory, Department of Microbiology, House on the Hill, Stobhill Hospital, Balornock Road, Glasgow, G21 3UW.
Mol Biotechnol. 2004 Oct;28(2):129-37. doi: 10.1385/mb:28:2:129.
Nucleotide sequencing is an established method for gaining information relating to partial gene, whole gene, or whole genome sequence. Here we describe some of the background leading to the advent of modern nucleotide sequencing and how it has led to the development of Pyrosequencing, a relatively new method for real-time nucleotide sequencing. In particular, we describe how this method can be used for typing bacterial pathogens.
核苷酸测序是一种获取与部分基因、全基因或全基因组序列相关信息的既定方法。在此，我们描述了导致现代核苷酸测序出现的一些背景情况，以及它如何促成了焦磷酸测序法的发展，焦磷酸测序法是一种相对较新的实时核苷酸测序方法。特别是，我们描述了该方法如何用于细菌病原体的分型。
