Rubtsova Myroslava, Kempe Katja, Gils Angelika, Ismagul Ainur, Weyen Jens, Gils Mario
Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) Gatersleben, Gatersleben, Germany.
Plant Cell Rep. 2008 Dec;27(12):1821-31. doi: 10.1007/s00299-008-0604-z. Epub 2008 Sep 17.
Site-specific recombination systems are becoming an important tool for the genetic modification of crop plants. Here we report the functional expression of the Streptomyces phage-derived phiC31 recombinase (integrase) in wheat. T-DNA constructs containing a phiC31 integrase transgene were stably transformed into wheat plants via particle gun bombardment. A plant-virus-based assay system was used to monitor the site-specific recombination activity of the recombinant integrase protein in vivo. We established several independent doubled haploid (DH) inbred lines that constitutively express an active integrase enzyme without any apparent detrimental effects on plant growth and development. The potential of phiC31 integrase expression in crop plants related to transgene control technologies or hybrid breeding systems is discussed.
位点特异性重组系统正成为作物遗传改良的重要工具。在此，我们报道了来源于链霉菌噬菌体的phiC31重组酶（整合酶）在小麦中的功能表达。含有phiC31整合酶转基因的T-DNA构建体通过粒子枪轰击稳定转化到小麦植株中。基于植物病毒的检测系统用于监测重组整合酶蛋白在体内的位点特异性重组活性。我们建立了几个独立的双单倍体（DH）自交系，这些自交系组成型表达活性整合酶，且对植物生长发育没有任何明显的有害影响。文中还讨论了phiC31整合酶在与转基因控制技术或杂交育种系统相关的作物中的表达潜力。
