Kohli Ajay, Twyman Richard M, Abranches Rita, Wegel Eva, Stoger Eva, Christou Paul
Rexagen Corporation, Genome Centre, Norwich Bio-Incubator, Colney Lane, Norwich, NR4 7UH, United Kingdom.
Plant Mol Biol. 2003 May;52(2):247-58. doi: 10.1023/a:1023941407376.
It has been appreciated for many years that the structure of a transgene locus can have a major influence on the level and stability of transgene expression. Until recently, however, it has been common practice to discard plant lines with poor or unstable expression levels in favor of those with practical uses. In the last few years, an increasing number of experiments have been carried out with the primary aim of characterizing transgene loci and studying the fundamental links between locus structure and expression. Cereals have been at the forefront of this research because molecular, genetic and cytogenetic analysis can be carried out in parallel to examine transgene loci in detail. This review discusses what is known about the structure and organization of transgene loci in cereals, both at the molecular and cytogenetic levels. In the latter case, important links are beginning to be revealed between higher order locus organization, nuclear architecture, chromatin structure and transgene expression.
多年来人们已经认识到，转基因位点的结构会对转基因表达的水平和稳定性产生重大影响。然而，直到最近，人们通常的做法还是舍弃那些表达水平差或不稳定的植物株系，而青睐具有实际用途的株系。在过去几年中，越来越多的实验以表征转基因位点并研究位点结构与表达之间的基本联系为主要目的而开展。谷类作物一直处于这项研究的前沿，因为可以同时进行分子、遗传和细胞遗传学分析，以详细检查转基因位点。本综述讨论了在分子和细胞遗传学水平上关于谷类作物中转基因位点的结构和组织的已知情况。在后一种情况下，高阶位点组织、核结构、染色质结构和转基因表达之间开始揭示出重要的联系。
