单核细胞增生李斯特菌产生一种通过ErbB2/ErbB3异二聚体发出信号的促侵袭因子。

Listeria monocytogenes produces a pro-invasive factor that signals via ErbB2/ErbB3 heterodimers.

作者信息

Oliveira Maria José, Lauwaet Tineke, De Bruyne Georges, Mareel Marc, Leroy Ancy

机构信息

Ghent University Hospital, De Pintelaan 185 P7, Ghent, Belgium.

出版信息

J Cancer Res Clin Oncol. 2005 Jan;131(1):49-59. doi: 10.1007/s00432-004-0601-8. Epub 2004 Oct 8.

DOI:10.1007/s00432-004-0601-8

PMID:15480783

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12161173/

Abstract

PURPOSE

We have previously demonstrated that conditioned medium from bacteria, some of which were isolated from the colon of cancer patients, stimulate cancer cell invasion in vitro through a 13-mer beta-casein-derived peptide. Since invasion signalling pathways are coordinated by the balance between protein kinases and phosphatases, we investigated the effect of conditioned medium from bacteria on the overall cellular tyrosine phosphorylation.

METHODS

The tyrosine phosphorylation level of HCT-8/E11 human colon cancer cells treated with the pro-invasive conditioned medium of Listeria, prepared on top of collagen type I gels (CM(Coll) Listeria/TSB), were analysed by means of immunoprecipitation and Western blot, with specific anti-phosphotyrosine antibodies.

RESULTS

We demonstrated that CM(Coll) Listeria/TSB increases the tyrosine phosphorylation level of ErbB2 and ErbB3, members of the epidermal growth factor receptor (EGFR) family, and the association between ErbB3 and the phosphatidylinositol 3-kinase (PI3K) regulatory subunit (p85alpha). CM(Coll) Listeria/TSB-stimulated ErbB3 tyrosine phosphorylation and cancer cell invasion were independent from EGFR expression and activity but dependent on ErbB2 activity.

CONCLUSIONS

The interaction between Listeria and collagen type I produces, next to the 13-mer peptide, at least another pro-invasive factor that signals via ErbB2/ErbB3 heterodimers.

摘要

目的

我们之前已经证明，来自细菌的条件培养基，其中一些是从癌症患者的结肠中分离出来的，可通过一种由13个氨基酸组成的β-酪蛋白衍生肽在体外刺激癌细胞侵袭。由于侵袭信号通路是由蛋白激酶和磷酸酶之间的平衡协调的，我们研究了细菌条件培养基对整体细胞酪氨酸磷酸化的影响。

方法

用在I型胶原凝胶上制备的侵袭性李斯特菌条件培养基（CM(Coll)李斯特菌/胰酪大豆胨肉汤）处理HCT-8/E11人结肠癌细胞，然后用特异性抗磷酸酪氨酸抗体通过免疫沉淀和蛋白质印迹法分析酪氨酸磷酸化水平。

结果

我们证明，CM(Coll)李斯特菌/胰酪大豆胨肉汤可增加表皮生长因子受体（EGFR）家族成员ErbB2和ErbB3的酪氨酸磷酸化水平，以及ErbB3与磷脂酰肌醇3激酶（PI3K）调节亚基（p85α）之间的关联。CM(Coll)李斯特菌/胰酪大豆胨肉汤刺激的ErbB3酪氨酸磷酸化和癌细胞侵袭与EGFR表达和活性无关，但依赖于ErbB2活性。