Liu Ning, Makino Toshiaki, Honda Gisho, Muso Eri, Kita Toru, Ono Takahiko
Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Clin Exp Nephrol. 2004 Sep;8(3):216-22. doi: 10.1007/s10157-004-0302-2.
Sairei-to (TJ-114) is a Japanese herbal medicine of standardized quality, originating from traditional Chinese medicine. In the present in vivo study, we investigated the suppressive effects of TJ-114, Shosaiko-to (TJ-9), and Saiboku-to (TJ-96) on mesangioproliferative glomerulonephritis (MsPGN) in rats. We evaluated the efficacy of these drugs on proteinuria, mesangial cell proliferation, and superoxide dismutase (SOD) activity.
MsPGN was induced in Wistar rats by intravenous injection of rabbit anti-rat thymocyte serum (ATS). TJ-114, TJ-9, or TJ-96 (500 mg/kg per day) was orally administered to the rats in drinking water from the day of ATS injection (day 0) to day 8, when rats were killed and kidney specimens were collected. The degree of mesangial cell proliferation was evaluated by immunostaining for proliferating cell nuclear antigen (PCNA) or macrophage antigen (ED-1). SOD activity in the homogenate of the renal cortex was also evaluated.
The amount of urinary albumin was significantly decreased only in the TJ-114-treated group compared with the disease control group ( P < 0.05). The number of PCNA- or ED-1-positive cells was significantly decreased in all the treatment groups ( P < 0.05, respectively, compared with the disease control group). SOD activity in the renal cortex homogenate was significantly augmented in all the treatment groups, most markedly in the TJ-96- and TJ-114-treated groups ( P < 0.01, respectively, compared with the disease control group).
These results suggest that TJ-114 suppresses the proliferation of mesangial cells through its antioxidative activity.
柴苓汤（TJ - 114）是一种质量标准化的日本草药，源自传统中药。在本次体内研究中，我们调查了柴苓汤、小柴胡汤（TJ - 9）和柴朴汤（TJ - 96）对大鼠系膜增生性肾小球肾炎（MsPGN）的抑制作用。我们评估了这些药物对蛋白尿、系膜细胞增殖和超氧化物歧化酶（SOD）活性的疗效。
通过静脉注射兔抗大鼠胸腺细胞血清（ATS）在Wistar大鼠中诱导MsPGN。从ATS注射当天（第0天）至第8天，将柴苓汤、小柴胡汤或柴朴汤（每天500 mg/kg）加入大鼠饮用水中口服给药，之后处死大鼠并收集肾脏标本。通过对增殖细胞核抗原（PCNA）或巨噬细胞抗原（ED - 1）进行免疫染色来评估系膜细胞增殖程度。还评估了肾皮质匀浆中的SOD活性。
与疾病对照组相比，仅柴苓汤治疗组的尿白蛋白量显著降低（P < 0.05）。所有治疗组中PCNA或ED - 1阳性细胞数量均显著减少（与疾病对照组相比，分别P < 0.05）。所有治疗组肾皮质匀浆中的SOD活性均显著增强，在柴朴汤和柴苓汤治疗组中最为明显（与疾病对照组相比，分别P < 0.01）。
这些结果表明，柴苓汤通过其抗氧化活性抑制系膜细胞增殖。
