Paul P S, Pomeroy K A, Muscoplat C C, Pomeroy B S, Sarma P S
Am J Vet Res. 1977 Mar;38(3):311-5.
Reticuloendotheliosis (RE) virus strains MN81 and MN67 isolated from epiornithics of RE in turkeys were partially characterized. Strains MN81 and MN67 replicated in chicken embryo fibroblast,duck embryo fibroblast and turkey embryo-fibroblast cultures and produced syncytial cytopathic effects in duck embryo fibroblast and turkey embryo fibroblast cultures. The virions of MN81 and MN67 measured approximately 100 nm in diameter, resembled RE virus strain T, and could be distinguished from avian leukosis viruses morphologically. The buoyant density of strain MN81 was found to be 1.15 g/cm3 in sucrose gradients. Strains MN81 and MN67 were inactivated by heat, acid pH, ether, and chloroform treatments. These strains were serologically unrelated to avian leukosis virus but were related to RE virus strains T, CS, DIA, and SN.
对从火鸡网状内皮组织增生症(RE)病鸟中分离出的RE病毒株MN81和MN67进行了部分特性鉴定。MN81和MN67毒株能在鸡胚成纤维细胞、鸭胚成纤维细胞和火鸡胚成纤维细胞培养物中复制,并在鸭胚成纤维细胞和火鸡胚成纤维细胞培养物中产生合胞体细胞病变效应。MN81和MN67的病毒粒子直径约为100纳米,类似于RE病毒株T,在形态上可与禽白血病病毒区分开来。在蔗糖梯度中发现MN81毒株的浮力密度为1.15克/立方厘米。MN81和MN67毒株经加热、酸性pH值、乙醚和氯仿处理后失活。这些毒株在血清学上与禽白血病病毒无关,但与RE病毒株T、CS、DIA和SN有关。