Chen P Y, Cui Z, Lee L F, Witter R L
Arch Virol. 1987;93(3-4):233-45. doi: 10.1007/BF01310977.
Antigenic relationships among 26 isolates of reticuloendotheliosis virus (REV) obtained from several avian species were compared by cross neutralization tests with polyclonal chicken sera and by immunofluorescent assays with monoclonal antibodies to REV strain T. The isolates were all strongly related by neutralization assays and thus probably constitute a single serotype. However, 3 antigenic subtypes were suggested by minor but distinct differences in neutralization titers. The validity of these 3 subtype designations was confirmed by differential reactivity of viral isolates to selected monoclonal antibodies. Subtype-associated differences in serum antibody titers were noted following the inoculation of chickens with the REV isolates.
通过用多克隆鸡血清进行交叉中和试验以及用针对网状内皮组织增生症病毒（REV）T株的单克隆抗体进行免疫荧光测定，比较了从几种禽类获得的26株REV之间的抗原关系。通过中和试验，这些分离株都具有很强的相关性，因此可能构成一个单一血清型。然而，中和效价存在微小但明显的差异，提示存在3种抗原亚型。病毒分离株对选定单克隆抗体的不同反应性证实了这3种亚型命名的有效性。给鸡接种REV分离株后，观察到血清抗体效价存在亚型相关差异。
