Smith E J, Solomon J J, Witter R L
Avian Dis. 1977 Oct-Dec;21(4):612-22.
A specific micro-complement-fixation procedure for assay of avian reticuloendotheliosis viruses (REV), designated by use as the COFAR test, was compared with an assay based on immunofluorescent (IF) antibody staining of infected chick embryo fibroblasts. Endpoint titrations in which REV strain T, chick syncytial virus, and spleen necrosis virus were used indicated that cultures infected with limiting dilutions of each strain were positive by both procedures within 6 days. Depending on cell density, infection of 2 to 9% of the cells cultured produced an unambiguous positive response (titer greater than or equal to 1:2) with COFAR test. When both tests were used in a study on the transmission and in vivo status of ducks infected with spleen necrosis virus, COFAR was no less, possibly more, sensitive than IF for detecting infection in cultures inoculated with plasma.
一种用于检测禽网状内皮组织增生症病毒（REV）的特定微量补体结合试验方法，命名为COFAR试验，与基于对感染鸡胚成纤维细胞进行免疫荧光（IF）抗体染色的检测方法进行了比较。使用REV T株、鸡多核细胞病毒和脾坏死病毒进行的终点滴定表明，用每种病毒的有限稀释液感染的培养物在6天内通过两种方法检测均呈阳性。根据细胞密度，培养的细胞中有2%至9%被感染时，COFAR试验会产生明确的阳性反应（效价大于或等于1:2）。当两种检测方法用于一项关于感染脾坏死病毒的鸭的传播和体内感染状况的研究时，在检测接种血浆的培养物中的感染情况方面，COFAR试验的敏感性不低于IF试验，甚至可能更高。
