Kang S Y, Nagaraja K V, Newman J A
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
Avian Dis. 1988 Apr-Jun;32(2):195-203.
Physical, chemical, and serological characterization of rotavirus isolates from turkeys was done. Cesium chloride (CsCl)-gradient isopycnic centrifugation of infected cell cultures revealed the presence of rotavirus particles of three different densities. They were double-shelled, single-shelled, and core particles. The double-shelled particles had a buoyant density (in CsCl) of 1.34 g/cml3, and that of single-shelled particles in CsCl was 1.36 g/cm3. The buoyant density of core particles in CsCl was greater than 1.40 g/cm3. These rotavirus isolates were not inactivated by chloroform and were relatively stable at pH 3.0. Their replication was not affected by 5-bromo-2'-deoxyuridine. Avian rotaviruses were not completely inactivated by heat treatment of 56 C for 8 hr. All six avian rotavirus isolates examined were antigenically related to each other. However, there was no antigenic relationship between mammalian rotaviruses and the avian rotavirus isolates examined.
对来自火鸡的轮状病毒分离株进行了物理、化学和血清学特征分析。对感染细胞培养物进行氯化铯（CsCl）梯度等密度离心，结果显示存在三种不同密度的轮状病毒颗粒。它们分别是双层壳颗粒、单层壳颗粒和核心颗粒。双层壳颗粒在CsCl中的浮力密度为1.34 g/cm³，单层壳颗粒在CsCl中的浮力密度为1.36 g/cm³。核心颗粒在CsCl中的浮力密度大于1.40 g/cm³。这些轮状病毒分离株不被氯仿灭活，在pH 3.0时相对稳定。它们的复制不受5-溴-2'-脱氧尿苷的影响。禽轮状病毒经56℃热处理8小时后并未完全灭活。所检测的所有六种禽轮状病毒分离株彼此之间存在抗原相关性。然而，哺乳动物轮状病毒与所检测的禽轮状病毒分离株之间不存在抗原关系。
