Bjarkam C R, Cancian G, Larsen M, Rosendahl F, Ettrup K S, Zeidler D, Blankholm A D, Østergaard L, Sunde N, Sørensen J C
Department of Neurobiology, Institute of Anatomy, University of Aarhus, DK-8000 Aarhus C, Denmark.
J Neurosci Methods. 2004 Oct 30;139(2):293-8. doi: 10.1016/j.jneumeth.2004.05.004.
We present a nonmagnetic Plexiglas stereotaxic localizer box that can be fitted directly to the pig skull by aluminum screws, allowing stereotaxic MRI or ventriculography and subsequent high-precision stereotaxic procedures. The localizer box was used to target the subthalamic nucleus (STN) bilaterally in five female Göttingen minipigs. Stereotaxic markers were inserted in the pig skull, the head fixated in the localizer box by aluminum screws inserted bilaterally in the zygoma bone with the hard palate locked on a horizontal palate holder. MRI was obtained on a 3T-MR-imager revealing the relation between the inserted markers and the estimated STN-position, and thus the target coordinates. After the MRI, a stereotaxic frame with attached micromanipulator was locked on to the localizer box converting it into a stereotaxic device. The stereotaxic markers were exposed and used as starting point for the stereotaxic procedure, whereby a microelectrode for electrolytic lesioning was inserted in the STN. Postmortem histological analysis revealed 70% correct STN-targeting. The average distance from the lesion center to the STN center was 1.2 mm with a S.D. of 1.1 mm. The most displaced lesion being 3.6 mm from the STN center. We conclude that the described localizer box secure firm head fixation, allowing stereotaxic MRI and subsequent conversion into a stereotaxic device for high-precision stereotaxic procedures.
我们展示了一种非磁性有机玻璃立体定位仪盒，它可以通过铝制螺丝直接安装在猪的头骨上，从而实现立体定位磁共振成像（MRI）或脑室造影以及后续的高精度立体定位手术。该定位仪盒用于对5只雌性哥廷根小型猪的双侧丘脑底核（STN）进行定位。在猪的头骨上插入立体定位标记物，通过双侧插入颧骨的铝制螺丝将头部固定在定位仪盒中，同时将硬腭锁定在水平腭托上。在3T磁共振成像仪上进行MRI检查，以揭示插入标记物与估计的STN位置之间的关系，从而确定目标坐标。MRI检查后，将带有连接微操作器的立体定位框架锁定在定位仪盒上，将其转换为立体定位设备。暴露立体定位标记物，并将其用作立体定位手术的起点，通过该起点将用于电解损伤的微电极插入STN。死后组织学分析显示STN定位的准确率为70%。从损伤中心到STN中心的平均距离为1.2毫米，标准差为1.1毫米。移位最大的损伤距离STN中心为3.6毫米。我们得出结论，所描述的定位仪盒能够确保头部牢固固定，允许进行立体定位MRI检查，并随后转换为用于高精度立体定位手术的立体定位设备。
