LeJeune J M, Hart L T, Larson A D, Seger C L
Am J Vet Res. 1977 Apr;38(4):459-63.
A microimmunodiffusion test (MIDT) specific for detection of antibodies to infectious bovine rhinotracheitis (IBR) in bovine serum has been developed. The antigen used in the MIDT was prepared from IBR virus-infected Madin-Darby bovine kidney cells grown in tissue culture. The antigen was stable, and relatively high yields were obtained readily. Results of the MIDT were obtained within 48 hours and agreed with those of the serum-neutralization test.
已开发出一种用于检测牛血清中传染性牛鼻气管炎（IBR）抗体的微量免疫扩散试验（MIDT）。MIDT中使用的抗原是由在组织培养中生长的感染IBR病毒的马迪-达比牛肾细胞制备的。该抗原稳定，且易于获得相对较高的产量。MIDT的结果在48小时内即可得出，且与血清中和试验的结果一致。
