Lupton H W, Reed D E
Am J Vet Res. 1980 Mar;41(3):383-90.
Subunit infectious bovine rhinotracheitis (IBR) antigens were prepared by solubilization of IBR-virus infected cell cultures with nonionic detergents Triton X-100 and NP-40. When used with Freund's incomplete adjuvant, Triton X-100 and NP-40 subunit antigens induced high serum-neutralization titers in vaccinated calves. Two doses of Triton X-100 and NP-40 subunit vaccines prevented the appearance of clinical signs of disease in all vaccinated calves exposed 30 days after the second vaccinal dose to intranasal challenge exposure with Cooper strain of IBR virus. Furthermore, two doses of NP-40 subunit vaccine prevented shedding of the virus in all vaccinated calves after challenge exposure.
通过用非离子去污剂Triton X-100和NP-40溶解感染传染性牛鼻气管炎（IBR）病毒的细胞培养物来制备亚单位抗原。当与弗氏不完全佐剂一起使用时，Triton X-100和NP-40亚单位抗原在接种疫苗的犊牛中诱导出高血清中和滴度。两剂Triton X-100和NP-40亚单位疫苗可防止所有在第二次接种疫苗剂量30天后经鼻用IBR病毒库珀株进行攻击暴露的接种疫苗犊牛出现疾病临床症状。此外，两剂NP-40亚单位疫苗可防止所有攻击暴露后的接种疫苗犊牛排出病毒。
