力对热力学和动力学的影响：单个RNA分子的解折叠
The effect of force on thermodynamics and kinetics: unfolding single RNA molecules.
作者信息
Tinoco I, Collin D, Li P T X
机构信息
Chemistry Department, University of California, Berkeley, CA 94720-1460, USA.
出版信息
Biochem Soc Trans. 2004 Nov;32(Pt 5):757-60. doi: 10.1042/BST0320757.
We have used laser tweezers to unfold single RNA molecules at room temperature and in physiological-type solvents. The forces necessary to unfold the RNAs are over the range 10-20 pN, forces that can be generated by cellular enzymes. The Gibbs free energy for the unfolding of TAR (transactivation-responsive) RNA from HIV was found to be increased after the addition of argininamide; the TAR hairpin was stabilized. The rate of unfolding was decreased and the rate of folding was increased by argininamide.
我们已使用激光镊子在室温及生理型溶剂中展开单个RNA分子。展开RNA所需的力在10 - 20皮牛顿范围内，这种力可由细胞酶产生。在添加精氨酰胺后，发现来自HIV的TAR（反式激活应答）RNA展开的吉布斯自由能增加；TAR发夹结构得到稳定。精氨酰胺降低了展开速率并提高了折叠速率。
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