Kemner Kenneth M, Kelly Shelly D, Lai Barry, Maser Joerg, O'loughlin Edward J, Sholto-Douglas Deirdre, Cai Zhonghou, Schneegurt Mark A, Kulpa Charles F, Nealson Kenneth H
Environmental Research Division and Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439-4843, USA.
Science. 2004 Oct 22;306(5696):686-7. doi: 10.1126/science.1103524.
High-energy x-ray fluorescence measurements were used to make elemental maps and qualitative chemical analyses of individual Pseudomonas fluorescens strain NCIMB 11764 cells. Marked differences between planktonic and adhered cells were seen in the morphology, elemental composition, and sensitivity to Cr(VI) of hydrated cells at spatial scales of 150 nm. This technology can be applied to natural geomicrobiological systems.
利用高能X射线荧光测量技术对荧光假单胞菌菌株NCIMB 11764的单个细胞进行元素图谱绘制和定性化学分析。在150纳米的空间尺度上，观察到浮游细胞和附着细胞在水合细胞的形态、元素组成以及对六价铬的敏感性方面存在显著差异。该技术可应用于自然地质微生物系统。
