Tomlinson C, Macintyre H, Dorrian C A, Ahmed S F, Wallace A M
Department of Child Health, Royal Hospital for Sick Children, Glasgow, Scotland, UK.
Arch Dis Child Fetal Neonatal Ed. 2004 Nov;89(6):F558-9. doi: 10.1136/adc.2003.034017.
Circulating testosterone concentrations in infants measured by a direct chemiluminescent immunoassay (Bayer ADVIA Centaur) were compared with those measured by a traditional radioimmunoassay using solvent extraction. The results confirm that neonatal circulating testosterone concentrations are method dependent, and each laboratory should establish method related reference ranges especially if using a direct commercial immunoassay. The results indicate that the Bayer ADVIA Centaur procedure can be used reliably in neonates. Expected values for male and female infants < 10 days old were 2.5-11.1 (n = 36) and 1.7-5.6 (n = 36) nmol/l respectively. For older neonates (10-50 days) the ranges were 0.2-17.2 (n = 42) and 0.1-1.5 (n = 7) nmol/l respectively.
采用直接化学发光免疫分析法（拜耳ADVIA Centaur）测量婴儿体内循环睾酮浓度，并与使用溶剂萃取的传统放射免疫分析法所测结果进行比较。结果证实，新生儿循环睾酮浓度取决于检测方法，每个实验室都应建立与方法相关的参考范围，特别是在使用直接商业免疫分析法时。结果表明，拜耳ADVIA Centaur检测程序可在新生儿中可靠使用。10日龄以下男婴和女婴的预期值分别为2.5 - 11.1（n = 36）和1.7 - 5.6（n = 36）nmol/l。对于较大的新生儿（10 - 50日龄），范围分别为0.2 - 17.2（n = 42）和0.1 - 1.5（n = 7）nmol/l。
