Conconi Maria Teresa, Lora Silvano, Baiguera Silvia, Boscolo Elisa, Folin Marcella, Scienza Renato, Rebuffat Piera, Parnigotto Pier Paolo, Nussdorfer Gastone Giovanni
Department of Pharmaceutical Sciences, University of Padua, Padua, Italy.
J Biomed Mater Res A. 2004 Dec 15;71(4):669-74. doi: 10.1002/jbm.a.30198.
Polyphosphazenes are polymers possessing a skeleton composed of alternating phosphorous and nitrogen atoms, and two side-moieties linked to each phosphorous atom. Polyphosphazenes with amino acid esters as side-moieties are biocompatible and biodegradable polymers. Two polyphosphazenes, poly[bis(ethyl alanate) phosphazene] and poly[(ethyl phenylalanate)0.8(ethyl alanate)0.8(ethyl glycinate)0.4 phosphazene] (PPAGP) were synthesized, and processed to form small fibers. Their ability to support rat neuromicrovascular endothelial cell (EC) adhesion and growth has been studied, using poly(D,L-lactic acid) as reference compound. Scanning electron microscopy revealed that both poly[bis(ethyl alanate) phosphazene] and PPAGP fibers were thinner than poly(D,L-lactic acid) fibers, and possessed a more irregular and porous surface. All polymers increased EC adhesion, compared with polystyrene, but only polyphosphazenes were able to improve EC growth. The highest increase in EC proliferation was induced by PPAGP, which, as revealed by environmental scanning electron microscopy, was also able to induce ECs to arrange into tubular structures. The conclusion is drawn that PPAGP may provide the best scaffold for engineered blood vessels, because it promotes adhesion, growth, and organization of ECs into capillary-like structures.
聚磷腈是一种聚合物，其骨架由交替的磷原子和氮原子组成，并且有两个侧基连接到每个磷原子上。以氨基酸酯为侧基的聚磷腈是具有生物相容性和可生物降解性的聚合物。合成了两种聚磷腈，聚[双（丙氨酸乙酯）磷腈]和聚[（苯丙氨酸乙酯）0.8（丙氨酸乙酯）0.8（甘氨酸乙酯）0.4磷腈]（PPAGP），并将其加工成小纤维。以聚（D，L-乳酸）作为参考化合物，研究了它们支持大鼠神经微血管内皮细胞（EC）黏附和生长的能力。扫描电子显微镜显示，聚[双（丙氨酸乙酯）磷腈]纤维和PPAGP纤维都比聚（D，L-乳酸）纤维细，并且具有更不规则和多孔的表面。与聚苯乙烯相比，所有聚合物都增加了EC的黏附，但只有聚磷腈能够促进EC的生长。PPAGP诱导EC增殖的增加最为显著，环境扫描电子显微镜显示，它还能诱导EC排列成管状结构。得出的结论是，PPAGP可能为工程血管提供最佳支架，因为它促进EC的黏附、生长以及将其组织成毛细血管样结构。
