Conconi Maria Teresa, Lora Silvano, Menti Anna Michela, Carampin Paolo, Parnigotto Pier Paolo
Department of Pharmaceutical Sciences, University of Padua, Padua, Italy.
Tissue Eng. 2006 Apr;12(4):811-9. doi: 10.1089/ten.2006.12.811.
Polyphosphazenes with amino acid ester as side groups are biocompatible polymers that could provide valid scaffolds for cell growth. In the present study we investigate the adhesion and growth of osteoblasts obtained from rat bone marrow on matrices composed of thin fibers of poly[bis(ethyl alanato)phosphazene] (PAlaP), poly(d,l-lactic acid) (PDLLA), or PAlaP/PDLLA blend. Our data show that scaffolds of PAlaP or PAlaP/PDLLA blend enhanced the cell adhesion and growth in comparison with that observed in cultures seeded on polystyrene tissue culture plates. Although collagenase-digestible protein synthesis remained unchanged, all scaffolds induced a decrease in alkaline phosphatase activity, suggesting that osteoblasts are in the proliferation phase. Both PAlaP and PAlaP blended with PDLLA may represent a new and interesting substrate for bone tissue engineering.
带有氨基酸酯侧基的聚磷腈是具有生物相容性的聚合物，可为细胞生长提供有效的支架。在本研究中，我们研究了从大鼠骨髓中获取的成骨细胞在由聚[双（丙氨酸乙酯）磷腈]（PAlaP）、聚（d，l-乳酸）（PDLLA）或PAlaP/PDLLA共混物的细纤维组成的基质上的黏附与生长情况。我们的数据表明，与接种在聚苯乙烯组织培养板上的培养物相比，PAlaP或PAlaP/PDLLA共混物的支架增强了细胞黏附与生长。尽管胶原酶可消化蛋白的合成保持不变，但所有支架均导致碱性磷酸酶活性降低，这表明成骨细胞处于增殖阶段。PAlaP以及与PDLLA共混的PAlaP都可能代表骨组织工程中一种新型且有趣的基质。
