Lavoie Holly A, McCoy George L, Blake Charles A
Department of Cell and Developmental Biology and Anatomy, School of Medicine, University of South Carolina Columbia, SC 29208, USA.
Mol Cell Endocrinol. 2004 Nov 30;227(1-2):31-40. doi: 10.1016/j.mce.2004.07.016.
Immunohistochemical studies were undertaken to determine the distribution of GATA-4 and GATA-6 in rat fetal gonad and the postnatal ovary during development and pregnancy. In the undifferentiated gonad, GATA-4 was expressed in the somatic cells of both sexes. After differentiation of the ovary and testis, GATA-4 expression continued in both ovarian and testicular somatic cells; whereas, GATA-6 was expressed in both somatic and germ cells. In the ovary of postnatal rats, granulosa and thecal cells of healthy follicles expressed both GATA factors. In the adult rat, GATA-4 expression was lower in corpora lutea as compared to follicles; whereas, GATA-6 was strongly expressed in both structures. GATA-4 expression was greater in functional corpora lutea than regressing corpora lutea. GATA-6 was expressed in both functional and regressing corpora lutea. In all postnatal ovaries, the expression of P450scc localized with tissue expressing GATA-4 and/or GATA-6, but GATA expression also occurred in P450scc negative cells.
进行免疫组织化学研究以确定GATA-4和GATA-6在大鼠胎儿性腺以及发育和妊娠期间产后卵巢中的分布。在未分化的性腺中，GATA-4在两性的体细胞中均有表达。卵巢和睾丸分化后，GATA-4在卵巢和睾丸的体细胞中持续表达；而GATA-6在体细胞和生殖细胞中均有表达。在产后大鼠的卵巢中，健康卵泡的颗粒细胞和膜细胞均表达这两种GATA因子。在成年大鼠中，与卵泡相比，黄体中GATA-4的表达较低；而GATA-6在这两种结构中均强烈表达。功能性黄体中GATA-4的表达高于退化黄体。GATA-6在功能性和退化黄体中均有表达。在所有产后卵巢中，P450scc的表达与表达GATA-4和/或GATA-6的组织定位一致，但GATA表达也出现在P450scc阴性细胞中。
