Liu Y, Broman J, Edvinsson L
Department of Internal Medicine, University Hospital, S-221 85 Lund, Sweden.
Neuroscience. 2004;129(2):431-7. doi: 10.1016/j.neuroscience.2004.07.045.
The central projections of the rat superior sagittal sinus (SSS) sensory innervation were studied by transganglionic tract tracing techniques. Cholera toxin subunit b (CTb) or wheat germ agglutinin-horseradish peroxidase conjugate (WGA-HRP) was applied on the overlying dura of the SSS and labeled terminations in the brainstem and cervical spinal cord were examined under the light microscope. Labeled cell bodies were seen bilaterally in the trigeminal ganglia and in the C2 dorsal root ganglia following both CTb and WGA-HRP applications. In the brainstem, labeled terminations were mainly found in the caudal and interpolar parts of the spinal trigeminal nucleus. In the CTb cases, terminations were also found in the dorsolateral part of the cuneate nucleus. In the spinal cord, labeled terminations were primarily located in the most ventrolateral part of the C1-C3 spinal dorsal horns on both sides. WGA-HRP labeled terminations were mainly located in laminae I and II, whereas CTb-labeled terminations located in laminae III and IV. These results indicate that the sensory information from the SSS is transmitted through both trigeminal and cervical spinal nerve branches to a primary sensory nervous center that extends from the C3 dorsal horn until to the interpolar part of the spinal trigeminal nucleus.
采用跨神经节追踪技术研究大鼠上矢状窦（SSS）感觉神经支配的中枢投射。将霍乱毒素亚基b（CTb）或麦胚凝集素 - 辣根过氧化物酶复合物（WGA - HRP）应用于SSS上方的硬脑膜，并在光学显微镜下检查脑干和颈脊髓中的标记终末。在应用CTb和WGA - HRP后，在三叉神经节和C2背根神经节双侧均可见标记的细胞体。在脑干中，标记的终末主要见于三叉神经脊束核的尾侧部和极间部。在CTb组中，在楔束核的背外侧部也发现了终末。在脊髓中，标记的终末主要位于双侧C1 - C3脊髓背角的最腹外侧部分。WGA - HRP标记的终末主要位于I层和II层，而CTb标记的终末位于III层和IV层。这些结果表明，来自SSS的感觉信息通过三叉神经和颈脊神经分支传递到一个从C3背角延伸至三叉神经脊束核极间部的初级感觉神经中枢。
