偏头痛雄性大鼠中导水管周围灰质和双侧三叉神经尾核之间的不对称调节的相关性研究。
A study on the correlation of the asymmetric regulation between the periaqueductal gray and the bilateral trigeminal nucleus caudalis in migraine male rats.
机构信息
The First School of Clinical Medicine of Zhejiang Chinese Medical University, Hangzhou, China.
Department of Radiology, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), 54 Youdian Road, Hangzhou, China.
出版信息
J Headache Pain. 2023 Mar 20;24(1):27. doi: 10.1186/s10194-023-01559-4.
BACKGROUND
The study was designed to explore the correlation of the asymmetric regulation between periaqueductal gray (PAG) and bilateral trigeminal nucleus caudalis (TNC) in migraine rats through studying the changes of metabolites in pain regulatory pathway of acute migraine attack.
METHODS
Thirty male Sprague-Dawley (SD) rats were randomly divided into three groups: blank, control, model groups. Then, blank group was intraperitoneally injected with ultrapure water, while control group injected with saline and model group injected with Glyceryl Trinitrate (GTN). Two hours later, PAG and bilateral TNC were removed respectively, and metabolite concentrations of PAG, Left-TNC, Right-TNC were obtained. Lastly, the differences of metabolite among three brain tissues were compared.
RESULTS
The relative concentrations of rNAA, rGlu, rGln, rTau, rMI in PAG or bilateral TNC had interaction effects between groups and sites. The concentration of rLac of three brain tissues increased in migraine rats, however, the rLac of LTNC and RTNC increased more than that of PAG. Besides, the concentrations of rNAA and rGln increased in RTNC, while rGABA decreased in RTNC.
CONCLUSIONS
There is correlation between PAG, LTNC and RTNC in regulation of pain during acute migraine attack, and the regulation of LTNC and RTNC on pain is asymmetric.
背景
本研究旨在通过研究急性偏头痛发作时疼痛调节通路中代谢物的变化，探讨偏头痛大鼠中导水管周围灰质（PAG）与双侧三叉神经尾核（TNC）之间不对称调节的相关性。
方法
30 只雄性 Sprague-Dawley（SD）大鼠随机分为三组：空白组、对照组和模型组。然后，空白组腹腔注射超纯水，对照组注射生理盐水，模型组注射甘油三硝酸酯（GTN）。2 小时后，分别取出 PAG 和双侧 TNC，获得 PAG、左 TNC、右 TNC 的代谢物浓度。最后，比较三种脑组织之间代谢物的差异。
结果
PAG 或双侧 TNC 中 rNAA、rGlu、rGln、rTau、rMI 的相对浓度存在组间和部位间的交互作用。偏头痛大鼠三种脑组织的 rLac 浓度均升高，但 LTNC 和 RTNC 的 rLac 浓度升高高于 PAG。此外，RTNC 中的 rNAA 和 rGln 浓度增加，而 rGABA 浓度降低。
结论
在急性偏头痛发作期间，PAG、LTNC 和 RTNC 之间存在相关性，LTNC 和 RTNC 对疼痛的调节呈不对称性。
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