Glover J N Mark, Williams R Scott, Lee Megan S
Department of Biochemistry, University of Alberta, Edmonton, AB, Canada T6G 2H7.
Trends Biochem Sci. 2004 Nov;29(11):579-85. doi: 10.1016/j.tibs.2004.09.010.
The C-terminal region of the breast-cancer-associated protein BRCA1 contains a pair of tandem BRCA1 C-terminal (BRCT) repeats that are essential for the tumour suppressor function of the protein. Similar repeat sequences have been identified in many proteins that seem to mediate cellular mechanisms for dealing with DNA damage. The BRCT domain in BRCA1 has been recently shown to constitute a module for recognizing phosphorylated (phospho-) peptides, with a recognition groove that spans both BRCT repeats. The fact that many other BRCT-containing proteins have phospho-peptide binding activity suggests that BRCT repeats might mediate phosphorylation-dependent protein-protein interactions in processes that are central to cell-cycle checkpoint and DNA repair functions.
乳腺癌相关蛋白BRCA1的C末端区域包含一对串联的BRCA1 C末端（BRCT）重复序列，这些重复序列对于该蛋白的肿瘤抑制功能至关重要。在许多似乎介导处理DNA损伤的细胞机制的蛋白质中也发现了类似的重复序列。最近研究表明，BRCA1中的BRCT结构域构成了一个识别磷酸化（磷酸化）肽段的模块，其识别凹槽跨越两个BRCT重复序列。许多其他含BRCT的蛋白质具有磷酸化肽段结合活性这一事实表明，BRCT重复序列可能在细胞周期检查点和DNA修复功能的核心过程中介导磷酸化依赖性蛋白质-蛋白质相互作用。
