Xue Tian, Stavropoulos Evangelos, Yang Min, Ragno Silvia, Vordermeier Martin, Chambers Mark, Hewinson Glyn, Lowrie Douglas B, Colston M Joseph, Tascon Ricardo E
Mycobacterial Division, National Institute for Medical Research, London NW7 1AA, UK.
Infect Immun. 2004 Nov;72(11):6324-9. doi: 10.1128/IAI.72.11.6324-6329.2004.
We have previously demonstrated that vaccination of mice with plasmid DNA vectors expressing immunodominant mycobacterial genes induced cellular immune responses and significant protection against challenge with Mycobacterium tuberculosis. We demonstrate here, using in vitro-synthesized RNA, that vaccination with DNA or RNA constructs expressing the M. tuberculosis MPT83 antigen are capable of inducing specific humoral and T-cell immune responses and confer modest but significant protection against M. tuberculosis challenge in mice. This is the first report of protective immunity conferred against intracellular bacteria by an RNA vaccine. This novel approach avoids some of the drawbacks of DNA vaccines and illustrates the potential for developing new antimycobacterial immunization strategies.
我们之前已经证明，用表达免疫显性分枝杆菌基因的质粒DNA载体对小鼠进行疫苗接种可诱导细胞免疫反应，并对结核分枝杆菌攻击提供显著保护。我们在此证明，使用体外合成的RNA，用表达结核分枝杆菌MPT83抗原的DNA或RNA构建体进行疫苗接种能够诱导特异性体液和T细胞免疫反应，并在小鼠中对结核分枝杆菌攻击提供适度但显著的保护。这是RNA疫苗针对细胞内细菌赋予保护性免疫的首次报道。这种新方法避免了DNA疫苗的一些缺点，并说明了开发新的抗分枝杆菌免疫策略的潜力。
