Wang Qing-min, Kang Lin, Wang Xiao-hua
The Ship Environmental Health Division, Institute of Navy Medicine Research, Xiangyin Road 880, Shanghai, China.
Microbiol Immunol. 2009 Jul;53(7):384-90. doi: 10.1111/j.1348-0421.2009.00138.x.
The present study evaluated the immune response elicited by a ubiquitin-fused ESAT-6 DNA vaccine against Mycobacterium tuberculosis. BALB/c mice were vaccinated with plasmid DNA encoding ESAT-6 protein, ubiquitin-fused ESAT-6 DNA vaccine (UbGR-ESAT-6), pcDNA3-ubiquitin and blank vector, respectively. ESAT-6 DNA vaccine immunization induced a Thl-polarized immune response. The production of Thl-type cytokine (IFN-gamma) and proliferative T-cell responses was enhanced significantly in mice immunized with UbGR-ESAT-6 fusion DNA vaccine, compared to non-fusion DNA vaccine. This fusion DNA vaccine also resulted in an increased relative ratio of IgG(2a) to IgG(l) and the cytotoxicity of T cells. Thus, the present study demonstrated that the UbGR-ESAT-6 fusion DNA vaccine inoculation improved antigen-specific cellular immune responses, which is helpful for protection against tuberculosis infection.
本研究评估了泛素融合的ESAT-6 DNA疫苗对结核分枝杆菌引发的免疫反应。分别用编码ESAT-6蛋白的质粒DNA、泛素融合的ESAT-6 DNA疫苗（UbGR-ESAT-6）、pcDNA3-泛素和空载体对BALB/c小鼠进行接种。ESAT-6 DNA疫苗免疫诱导了Th1极化的免疫反应。与非融合DNA疫苗相比，用UbGR-ESAT-6融合DNA疫苗免疫的小鼠中，Th1型细胞因子（IFN-γ）的产生和增殖性T细胞反应显著增强。这种融合DNA疫苗还导致IgG(2a)与IgG(l)的相对比例增加以及T细胞的细胞毒性增加。因此，本研究表明，接种UbGR-ESAT-6融合DNA疫苗可改善抗原特异性细胞免疫反应，这有助于预防结核感染。
