巴伯-斯托纳-凯利培养基的变化会调节伯氏疏螺旋体临床分离株的感染性和致病性。
Variations in Barbour-Stoenner-Kelly culture medium modulate infectivity and pathogenicity of Borrelia burgdorferi clinical isolates.
作者信息
Wang Guiqing, Iyer Radha, Bittker Susan, Cooper Denise, Small Jennifer, Wormser Gary P, Schwartz Ira
机构信息
Department of Microbiology & Immunology, New York Medical College, Valhalla 10595, USA.
出版信息
Infect Immun. 2004 Nov;72(11):6702-6. doi: 10.1128/IAI.72.11.6702-6706.2004.
Abstract
The effects of variations in Barbour-Stoenner-Kelly (BSK) medium on the infectivity and pathogenicity of Borrelia burgdorferi clinical isolates were assessed by retrospective and prospective studies using a murine model of Lyme borreliosis. Thirty of 35 (86%) mice infected with any of six virulent B. burgdorferi clinical isolates grown in a BSK-H medium developed clinically apparent arthritis. By contrast, arthritis was observed in only 25 of 60 (42%) mice inoculated with two of these B. burgdorferi strains grown in a different lot of BSK-H medium (P < 0.001). In a prospective study, mice inoculated with a B. burgdorferi clinical isolate grown in a BSK medium prepared in-house produced significantly greater disease than those injected with the same isolate cultured in BSK-H medium (P < 0.05). The attenuated pathogenicity is not due to the loss of plasmids during in vitro cultivation. The data suggest that variations in BSK medium have a significant impact on the infectivity and pathogenicity of B. burgdorferi clinical isolates.
摘要
采用莱姆病螺旋体病小鼠模型，通过回顾性和前瞻性研究，评估了巴伯-斯托纳-凯利（BSK）培养基的变化对伯氏疏螺旋体临床分离株的感染性和致病性的影响。用在BSK-H培养基中培养的6种强毒力伯氏疏螺旋体临床分离株中的任何一种感染小鼠，35只中有30只（86%）出现了明显的临床关节炎。相比之下，接种在另一批BSK-H培养基中培养的这两种伯氏疏螺旋体菌株的60只小鼠中，只有25只（42%）出现了关节炎（P<0.001）。在一项前瞻性研究中，接种在内部制备的BSK培养基中培养的伯氏疏螺旋体临床分离株的小鼠，比接种在BSK-H培养基中培养的相同分离株的小鼠产生的疾病明显更严重（P<0.05）。致病性减弱并非由于体外培养过程中质粒的丢失。数据表明，BSK培养基的变化对伯氏疏螺旋体临床分离株的感染性和致病性有显著影响。
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