Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, 80521, USA.
Texas A&M AgriLife Research, Uvalde, TX, 78801, USA.
Sci Rep. 2021 Jan 21;11(1):1926. doi: 10.1038/s41598-021-81252-1.
Borrelia spirochetes are the causative agents of Lyme borreliosis (LB) and relapsing fever (RF). Despite the steady rise in infections and the identification of new species causing human illness over the last decade, isolation of borreliae in culture has become increasingly rare. A modified Barbour-Stoenner-Kelly (BSK) media formulation, BSK-R, was developed for isolation of the emerging RF pathogen, Borrelia miyamotoi. BSK-R is a diluted BSK-II derivative supplemented with Lebovitz's L-15, mouse and fetal calf serum. Decreasing the concentration of CMRL 1066 and other components was essential for growth of North American B. miyamotoi. Sixteen B. miyamotoi isolates, originating from Ixodes scapularis ticks, rodent and human blood collected in the eastern and upper midwestern United States, were isolated and propagated to densities > 10 spirochetes/mL. Growth of five other RF and ten different LB borreliae readily occurred in BSK-R. Additionally, primary culture recovery of 20 isolates of Borrelia hermsii, Borrelia turicatae, Borrelia burgdorferi and Borrelia mayonii was achieved in BSK-R using whole blood from infected patients. These data indicate this broadly encompassing borreliae media can aid in in vitro culture recovery of RF and LB spirochetes, including the direct isolation of new and emerging human pathogens.
螺旋体是莱姆病（LB）和回归热（RF）的病原体。尽管在过去十年中，感染病例稳步上升，并且发现了新的致病物种，但在培养中分离出螺旋体变得越来越罕见。改良的 Barbour-Stoenner-Kelly（BSK）培养基配方 BSK-R 是为分离新兴 RF 病原体伯氏疏螺旋体而开发的。BSK-R 是 BSK-II 的稀释衍生产品，添加了 Lebovitz 的 L-15、鼠和胎牛血清。降低 CMRL 1066 和其他成分的浓度对于北美伯氏疏螺旋体的生长至关重要。从美国东部和中西部采集的硬蜱、啮齿动物和人血中分离出 16 株伯氏疏螺旋体，在 BSK-R 中培养至密度>10 个螺旋体/mL。BSK-R 中还容易生长五种其他 RF 和十种不同的 LB 螺旋体。此外，使用感染患者的全血在 BSK-R 中成功进行了 20 株赫尔姆斯氏疏螺旋体、土耳其疏螺旋体、伯氏疏螺旋体和梅奥尼亚疏螺旋体的原代培养回收。这些数据表明，这种广泛包含螺旋体的培养基可以帮助体外培养 RF 和 LB 螺旋体，包括直接分离新出现的人类病原体。
