从小鼠中收获和检测体外克隆形成角质形成干细胞的方法。
Method for the harvest and assay of in vitro clonogenic keratinocytes stem cells from mice.
作者信息
Wu Wei-Yang, Morris Rebecca J
机构信息
Department of Dermatology, Columbia University College of Physicians and Surgeons, New York, NY, USA.
出版信息
Methods Mol Biol. 2005;289:79-86. doi: 10.1385/1-59259-830-7:079.
Primary adult mouse keratinocytes are harvested from the dorsal section of 6- to 8-wk-old mice. Euthanized mice are clipped and sterilized with serial washes in provodine iodine and ethanol solutions. The skin of the mice is removed and treated with trypsin to detach the dermis from the epidermis. Cells harvested from this proven method can be used for molecular biology, biochemistry, or a variety of cell culture techniques, including the clonogenic cell assay presented here.
原代成年小鼠角质形成细胞取自6至8周龄小鼠的背部皮肤。对安乐死的小鼠进行剪毛处理，并用碘伏和乙醇溶液多次冲洗进行消毒。去除小鼠的皮肤，用胰蛋白酶处理以使真皮与表皮分离。通过这种经过验证的方法收获的细胞可用于分子生物学、生物化学或各种细胞培养技术，包括本文介绍的克隆形成细胞测定。
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