Gilani Anwarul Hassan, Ghayur M Nabeel
Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, Karachi 74800, Pakistan.
J Ethnopharmacol. 2004 Dec;95(2-3):169-72. doi: 10.1016/j.jep.2004.06.038.
The crude extract of Raphanus sativus leaves (Rl.Cr) showed a dose-dependent (0.03-5.0 mg/ml) spasmogenicity in guinea-pig ileum and colon. The effect was insensitive to atropine pre-treatment but was completely abolished by pyrilamine indicating involvement of histaminergic (H(1)) receptors. The contractile effect at high doses (3.0-5.0mg/ml) was followed by relaxation. Rl.Cr also enhanced the transit of charcoal meal in mice at 30-100 mg/kg. The petroleum spirit, chloroform and aqueous fractions all showed histaminergic activity in ileum; aqueous fraction being more potent. The study shows the presence of a histaminergic component(s) along with a weak spasmolytic factor thus providing sound mechanistic basis for the traditional use of the plant in constipation.
萝卜叶粗提物（Rl.Cr）在豚鼠回肠和结肠中呈现剂量依赖性（0.03 - 5.0毫克/毫升）的致痉挛性。该效应对阿托品预处理不敏感，但可被吡苄明完全消除，表明组胺能（H(1)）受体参与其中。高剂量（3.0 - 5.0毫克/毫升）时的收缩效应之后会出现松弛。Rl.Cr在30 - 100毫克/千克剂量下还能促进小鼠炭末推进。石油醚、氯仿和水相部分在回肠中均显示出组胺能活性；水相部分活性更强。该研究表明存在组胺能成分以及一种微弱的解痉因子，从而为该植物在便秘传统应用中提供了合理的作用机制依据。
